Metabolic alkalosis reduces exercise-induced acidosis and potassium accumulation in human skeletal muscle interstitium
Metabolic alkalosis reduces exercise-induced acidosis and potassium accumulation in human skeletal muscle interstitium
复制标题
DOI：
10.1113/jphysiol.2005.086801
复制
发表时间：
2005-07-15
影响因子：
5.5
通讯作者：
Juel, C
中科院分区：
文献类型：
--
作者：
Street, D;Nielsen, JJ;Juel, C
骨骼肌在活动过程中释放钾。间质钾积累对于肌肉功能和运动引起的疲劳的发展是重要的。在本研究中，我们使用柠檬酸钠的摄入作为一种工具，以调查运动期间间质H+浓度和K+积累之间的关系。七名健康受试者在两个单独的日子进行单腿膝伸肌运动，有和没有柠檬酸钠摄入。用微透析技术测定细胞间质H+和K+浓度。柠檬酸盐摄入降低了血浆H+浓度，增加了血浆HCO 3-浓度。柠檬酸盐对静息时的间质H+无影响。与对照组相比，柠檬酸盐摄入组在剧烈运动期间间质H+浓度的增加显著较低（P < 0.05）（峰值间质H+浓度79 vs 131 nM）。运动3分钟后，与对照实验相比，柠檬酸盐组（paclisis）的间质K+浓度降低（P < 0.05）（8.0 +/- 0.9 vs 11.0 +/- 2 mm），并且间质K+浓度在其余运动期间保持较低。目前的研究表明，间质H+和K+积累之间的联系，这可能是通过ATP敏感的K+通道（K-ATP通道），这是敏感的H+的变化。
Skeletal muscle releases potassium during activity. Interstitial potassium accumulation is important for muscle function and the development of fatigue resulting from exercise. In the present study we used sodium citrate ingestion as a tool to investigate the relationship between interstitial H+ concentration and K+ accumulation during exercise. Seven healthy subjects performed one-legged knee-extensor exercise on two separate days with and without sodium citrate ingestion. Interstitial H+ and K+ concentrations were measured with the microdialysis technique. Citrate ingestion reduced the plasma H+ concentration and increased the plasma HCO3- concentration. Citrate had no effect on interstitial H+ at rest. The increase in interstitial H+ concentration during intense exercise was significantly lower (P < 0.05) with citrate ingestion compared to control (peak interstitial H+ concentration 79 versus 131 nM). After 3 min of exercise interstitial K+ concentration was reduced (P < 0.05) in the citrate (alkalosis) compared to the control experiment (8.0 +/- 0.9 versus 11.0 +/- 2 mm) and interstitial K+ concentration remained lower during the rest of the exercise period. The present study demonstrated a link between interstitial H+ and K+ accumulation, which may be through the ATP-sensitive K+ channels (K-ATP channels), which are sensitive to changes in H+.