Light regulation on growth, development, and secondary metabolism of marine-derived filamentous fungi
Light regulation on growth, development, and secondary metabolism of marine-derived filamentous fungi
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光对海洋丝状真菌生长、发育和次生代谢的调控
DOI：
10.1007/s12223-013-0242-x
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发表时间：
2013-11-01
影响因子：
2.6
通讯作者：
Zhang, Yuanxing
中科院分区：
文献类型：
--
作者：
Cai, Menghao;Fang, Zhe;Zhang, Yuanxing
研究了不同光照条件对3种海洋丝状真菌生长发育和次生代谢的影响。黑暗刺激灰绿曲霉HB 1 -19的有性发育，而白色、红色和蓝色光促进其无性行为。红光和蓝光促进了Xylariasp的无性子座形成。(no. 2508），但黑暗和白色光抑制其生长。(no. 1403）被证明对任何测试的光照射不敏感。根据实验数据，没有观察到发育与次生代谢之间的规律性。然而，真菌的生长表现出负相关的主要生物活性化合物（曲霉素A，1403 C，和木糖缩酮B）从各种菌株的生产。结果表明，曲霉素A的生物合成倾向于蓝光照射，而1403 C和木缩酮B的生物合成倾向于红光照射。在适宜的光照条件下，曲霉素A、1403 C和木糖缩酮B的产量分别比黑暗条件下提高了32.9%、21.9%和30.8%.对A. glaucusHB 1-19与其它系统的比较表明，A.灰绿菌HB 1-19与黄曲霉（Escherichgillusspp.）特别是A. nidulansin，尽管它的海洋来源的真菌的作用。这表明海洋真菌在适应海洋环境的过程中可能会保存其光响应系统。这项工作也提供了有用的信息，过程优化涉及光调控的生长和代谢的候选药物生产从光敏海洋真菌。
Effects of different light conditions on development, growth, and secondary metabolism of three marine-derived filamentous fungi were investigated. Darkness irritated sexual development ofAspergillus glaucusHB1-19, while white, red, and blue lights improved its asexual behavior. The red and blue lights improved asexual stroma formation ofXylariasp. (no. 2508), but the darkness and white light inhibited it. Differently, development ofHaloroselliniasp. (no. 1403) turned out to be insensitive to any tested light irradiation. Upon the experimental data, no regularity was observed linking development with secondary metabolism. However, fungal growth showed inversely correlation with productions of major bioactive compounds (aspergiolide A, 1403C, and xyloketal B) from various strains. The results indicated that aspergiolide A biosynthesis favored blue light illumination, while 1403C and xyloketal B preferred red light irradiation. With the favorite light sensing conditions, productions of aspergiolide A, 1403C, and xyloketal B were enhanced by 32.9, 21.9, and 30.8 % compared with those in the dark, respectively. The phylogenetic analysis comparing the light-responding proteins ofA. glaucusHB 1-19 with those in other systems indicated thatA. glaucusHB 1-19 was closely related toAspergillusspp. especiallyA. nidulansin spite of its role of marine-derived fungus. It indicated that marine fungi might conserve its light response system when adapting the marine environment. This work also offers useful information for process optimization involving light regulation on growth and metabolism for drug candidate production from light-sensitive marine fungi.