Analysis of circulating human and viral microRNAs in patients with congenital cytomegalovirus infection
Analysis of circulating human and viral microRNAs in patients with congenital cytomegalovirus infection
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DOI：
10.1038/jp.2016.157
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发表时间：
2016-12-01
影响因子：
2.9
通讯作者：
Ito，Y.
中科院分区：
文献类型：
--
作者：
Kawano，Y.;Kawada，J.;Ito，Y.
目的：巨细胞病毒（CMV）是先天性感染的最常见原因，可导致神经发育障碍，尽管大多数患者无症状。与疾病严重程度相关的生物标志物将是理想的，以区分无症状和可能从抗病毒治疗中获益的轻度症状患者。MicroRNAs（miRNAs）是一种非编码RNA，可能具有作为生物标志物的潜力。研究设计：13名先天性CMV感染的婴儿入选，通过实时PCR定量检测11种人类和3种CMV编码的miRNAs的血浆水平。结果：先天性巨细胞病毒感染患儿血浆中miR-183- 5 p和miR-210- 3 p水平显著高于对照组，但与临床特征无显著相关性。结论：血浆中miR-183- 5 p和miR-210- 3 p水平可能与先天性巨细胞病毒感染的发病机制有关，可作为疾病的生物标志物。
Objective:Cytomegalovirus (CMV) is the most common cause of congenital infection and can cause neurodevelopmental disabilities, although a majority of patients are asymptomatic. Biomarkers associated with disease severity would be desirable to distinguish asymptomatic from mildly symptomatic patients who may benefit from antiviral treatment. MicroRNAs (miRNAs) are noncoding RNAs that may have the potential to serve as biomarkers.Study Design:Thirteen infants with congenital CMV infection were enrolled, and plasma levels of 11 human-and 3 CMV-encoded miRNAs were quantitated by real-time PCR. Plasma levels of miRNAs and their associations with clinical features were evaluated.Results:The levels of miR-183-5p and miR-210-3p were significantly higher in patients with congenital CMV infection than in control infants, whereas no significant associations between levels of miRNAs and clinical features of congenital CMV infection were observed.Conclusion:Plasma miRNAs could be associated with the pathogenesis of congenital CMV infection and could be used as disease biomarkers.