In vitro effects of psychoactive and non-psychoactive cannabinoids on immature rat Sertoli cell function.
In vitro effects of psychoactive and non-psychoactive cannabinoids on immature rat Sertoli cell function.
复制标题
精神活性和非精神活性大麻素对未成熟大鼠支持细胞功能的体外影响。
DOI：
10.1016/0024-3205(93)90585-q
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发表时间：
1993
期刊：
影响因子：
6.1
通讯作者：
Bartke,A
中科院分区：
文献类型：
--
作者：
Newton,SC;Murphy,LL;Bartke,A
我们研究了精神活性和非精神活性大麻素对分离的未成熟大鼠支持细胞在无血清或含血清培养基中培养的影响。无血清对照培养中支持细胞的乳酸积累比暴露于血清对照培养中获得的值高10倍。在两种培养条件下，3.1 μg/ml的δ -9-四氢大麻酚（THC）或大麻酚（CBN）刺激乳酸分泌高于对照水平。大麻二酚（CBD）在含血清培养基中刺激乳酸分泌，而在无血清培养基中没有。在无血清培养条件下，我们研究了四氢大麻酚和肾上腺素或卵泡刺激素联合使用对未成熟大鼠Sertoli细胞分泌乳酸和转铁蛋白的影响。0.1 μM肾上腺素与0.8或3.1 μg/ml四氢大麻酚共孵育，与单独使用肾上腺素或四氢大麻酚相比，显著刺激乳酸分泌，只有高剂量四氢大麻酚才会增加转铁蛋白分泌。此外，FSH （1 μg/ml）与THC（0.8或3.1 μg/ml）共孵育可显著刺激未成熟大鼠Sertoli细胞产生乳酸和转铁蛋白。这些结果增加了大麻素可以对支持细胞功能产生直接影响并调节其对生理刺激的反应的证据。
We have examined the effects of psychoactive and non-psychoactive cannabinoids on isolated immature rat Sertoli cells cultured in either serum-free or serum-containing media. Lactate accumulation by Sertoli cells in serum-free control cultures was 10 fold greater than the values obtained in control cultures exposed to serum. Under either culture condition, 3.1 μg/ml of delta-9-tetrahydrocannabinol (THC) or cannabinol (CBN) stimulated lactate secretion above control levels. Cannabidiol (CBD) stimulated lactate secretion in serum-containing but not in serum-free media. Using serum-free culture conditions, we next studied the in vitro effects of combinations of THC and either epinephrine or FSH on lactate and transferrin secretion by immature rat Sertoli cells. Co-incubation of 0.1 μM epinephrine with 0.8 or 3.1 μg/ml THC significantly stimulated lactate secretion when compared to epinephrine or THC alone, while only the high THC dose increased transferrin secretion. Moreover, co-incubation of FSH (1 μg/ml) with THC (0.8 or 3.1 μg/ml), significantly stimulated both lactate and transferrin production by immature rat Sertoli cells. These results add to the growing evidence that cannabinoids can exert direct effects on Sertoli cell function and modulate their responses to physiological stimuli.