Chromosomal Arrangement of Phosphorelay Genes Couples Sporulation and DNA Replication.
Chromosomal Arrangement of Phosphorelay Genes Couples Sporulation and DNA Replication.
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DOI：
10.1016/j.cell.2015.06.012
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发表时间：
2015-07-16
期刊：
影响因子：
64.5
通讯作者：
Igoshin OA
中科院分区：
文献类型：
--
作者：
Narula J;Kuchina A;Lee DD;Fujita M;Süel GM;Igoshin OA
在共同调节网络中编码蛋白质的基因通常位于染色体上彼此靠近，以促进共同调节或将基因表达与生长速度偶联。与这些观察结果相比，这里我们证明了枯草芽孢杆菌孢子网络基因在染色体相对侧的排列的功能作用。我们发现两个孢子网络基因的排列，一个位于原点附近，另一个靠近末端，导致染色体复制过程中短暂的基因剂量失衡。这种不平衡被产孢网络检测到，从而产生产孢主调控因子Spo0A~P的细胞周期协调脉冲。这种脉冲反应使细胞在饥饿中度过的每个细胞周期中决定是产孢还是继续营养生长。这种协调机制的简单性表明它可能广泛适用于各种基因调控和应激反应设置。
Genes encoding proteins in a common regulatory network are frequently located close to one another on the chromosome to facilitate co-regulation or couple gene expression to growth rate. Contrasting with these observations, here we demonstrate a functional role for the arrangement of Bacillus subtilis sporulation network genes on opposite sides of the chromosome. We show that the arrangement of two sporulation network genes, one located close to the origin, the other close to the terminus leads to a transient gene dosage imbalance during chromosome replication. This imbalance is detected by the sporulation network to produce cell-cycle coordinated pulses of the sporulation master regulator Spo0A~P. This pulsed response allows cells to decide between sporulation and continued vegetative growth during each cell-cycle spent in starvation. The simplicity of this coordination mechanism suggests that it may be widely applicable in a variety of gene regulatory and stress-response settings.