Simvastatin enhanced antimicrobial effect of Ag+againstE. faecalisinfection of dentine through PLGA co-delivery submicron particles
Simvastatin enhanced antimicrobial effect of Ag+againstE. faecalisinfection of dentine through PLGA co-delivery submicron particles
复制标题
辛伐他汀通过 PLGA 共传递亚微米颗粒增强 Ag( ) 对牙本质粪肠球菌感染的抗菌作用。
DOI：
10.1080/09205063.2020.1811188
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发表时间：
2020-09-01
影响因子：
3.6
通讯作者：
Fan, Bing
中科院分区：
文献类型：
--
作者：
Fan, Wei;Duan, Mengting;Fan, Bing
粪肠球菌（E. faecalis）是牙齿难治性感染的优势菌之一。银离子（Ag+）已被证明是一种强杀菌剂，但具有高细胞毒性和变色性。辛伐他汀是一种用于治疗血脂异常的药物，具有抗炎特性。本研究首次将Ag(+)和辛伐他汀联合使用，并制备了同时携带Ag(+)和辛伐他汀的聚丙交酯-乙交酯(PLGA)亚微米颗粒(AgS-PLGA)以供进一步研究。结果证实了对大肠杆菌的抗菌活性增强。辛伐他汀粪Ag(+)。 AgS-PLGA可同时释放Ag(+)和辛伐他汀24天，并且抗菌活性增强。在牙本质切片上，AgS-PLGA可以通过超声激活进入牙本质小管并抑制大肠杆菌的定植。粪肠球菌。 AgS-PLGA对MC3T3-E1细胞无细胞毒性，对RAW-264.7细胞有轻微抑制作用，并能减少RAW-264.7细胞IL-6和IL-1β的分泌。 AgS-PLGA 可以开发为一种新的生物材料，用于牙科和相关医疗治疗的感染和炎症控制。
Enterococcus faecalis (E. faecalis)is one of the dominant bacteria for refractory infections of teeth. Silver ions (Ag+) have been proved to be a strong bactericide but with high cytotoxicity and discoloration property. Simvastatin is an agent used for dyslipidemia treatment and has anti-inflammatory property. In this study, Ag(+)and simvastatin were for the first time used in combination, and poly (lactide-co-glycolide) (PLGA) submicron particles carrying both Ag(+)and simvastatin (AgS-PLGA) were fabricated for further investigations. Results confirmed the enhanced antibacterial activity againstE. faecalisof Ag(+)by simvastatin. AgS-PLGA could release both Ag(+)and simvastatin for 24 days and also showed enhanced antibacterial activities. On dentin slices, AgS-PLGA could enter dentinal tubules by ultrasonic activation and inhibit the colonization ofE. faecalis. AgS-PLGA showed no cytotoxicity on MC3T3-E1 cells and slight suppressive effect on RAW-264.7 cells, and could reduce the secretion of IL-6 and IL-1 beta of RAW-264.7 cells. AgS-PLGA could be developed as a new biomaterial for infection and inflammation control for dental and related medical treatments.