Carbon Dots Conjugated with Vascular Endothelial Growth Factor for Protein Tracking in Angiogenic Therapy
Carbon Dots Conjugated with Vascular Endothelial Growth Factor for Protein Tracking in Angiogenic Therapy
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DOI：
10.1021/acs.langmuir.9b03980
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发表时间：
2020-03-24
期刊：
影响因子：
3.9
通讯作者：
Kumacheva, Eugenia
中科院分区：
文献类型：
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作者：
Basiri, Hamideh;Mehrizi, Ali Abouei;Kumacheva, Eugenia
使用生长因子进行组织再生的挑战之一是监测其生物分布和递送至受损组织以进行微创检测。在本研究中，通过将人类血管内皮生长因子（VEGF）与光致发光碳点（CD）化学连接来实现追踪。通过水热法合成碳点，随后使用碳二亚胺偶联与 VEGF 结合。 ELISA 和蛋白质印迹分析表明，VEGF 结合的 CD 保留了 VEGF 与其抗体的结合亲和力。我们还表明，VEGF 缀合的 CD 维持了 VEGF 对于管形成和细胞迁移的功能。 VEGF 结合的 CD 也用于人脐静脉内皮细胞的体外成像。这项工作的结果表明，细胞穿透性 VEGF 缀合 CD 可用于治疗和组织工程应用中的生长因子蛋白追踪。
One of the challenges of using growth factors for tissue regeneration is to monitor their biodistributions and delivery to injured tissues for minimally invasive detection. In the present study, tracking of human vascular endothelial growth factor (VEGF) was achieved by chemically linking it to photoluminescent carbon dots (CDs). Carbon dots were synthesized by the hydrothermal method and, subsequently, conjugated with VEGF using carbodiimide coupling. ELISA and western blot analysis revealed that VEGF-conjugated CDs preserve the binding affinity of VEGF to its antibodies. We also show that VEGF-conjugated CDs maintain the functionality of VEGF for tube formation and cell migration. The VEGF-conjugated CDs were also used for in vitro imaging of human umbilical vein endothelial cells. The results of this work suggest that cell-penetrating VEGF-conjugated CDs can be used for growth factor protein tracking in therapeutic and tissue engineering applications.