Site-specific vibrational dynamics of the CD3ζ membrane peptide using heterodyned two-dimensional infrared photon echo spectroscopy
Site-specific vibrational dynamics of the CD3ζ membrane peptide using heterodyned two-dimensional infrared photon echo spectroscopy
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DOI：
10.1063/1.1718332
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发表时间：
2004-06-01
影响因子：
4.4
通讯作者：
Zanni, MT
中科院分区：
文献类型：
--
作者：
Mukherjee, P;Krummel, AT;Zanni, MT
外差二维红外 (2D IR) 光谱已用于研究脂质囊泡中包含 T 细胞受体 CD3zeta 跨膜结构域的 27 个残基肽的酰胺 I 振动动力学。使用 1-C-13=O-18 同位素标记，对 49-亮氨酸残基的酰胺 I 模式进行光谱分离，并通过拟合用光子回波脉冲序列收集的 2D IR 光谱来测量该模式的均匀和非均匀线宽。纯相移线宽和非均匀线宽分别为 2 和 32 cm(-1)。酰胺 I 带的布居弛豫时间是用瞬态光栅测量的，它对线宽的贡献为 9 cm-1。 49-亮氨酸酰胺 I 模式和整个 CD3zeta 肽的酰胺 I 带的比较表明，沿肽长度的振动动力学并不均匀。讨论了 49-亮氨酸位点存在大量不均匀性的可能根源。 (C) 2004 年美国物理研究所。
Heterodyned two-dimensional infrared (2D IR) spectroscopy has been used to study the amide I vibrational dynamics of a 27-residue peptide in lipid vesicles that encompasses the transmembrane domain of the T-cell receptor CD3zeta. Using 1-C-13=O-18 isotope labeling, the amide I mode of the 49-Leucine residue was spectroscopically isolated and the homogeneous and inhomogeneous linewidths of this mode were measured by fitting the 2D IR spectrum collected with a photon echo pulse sequence. The pure dephasing and inhomogeneous linewidths are 2 and 32 cm(-1), respectively. The population relaxation time of the amide I band was measured with a transient grating, and it contributes 9 cm-1 to the linewidth. Comparison of the 49-Leucine amide I mode and the amide I band of the entire CD3zeta peptide reveals that the vibrational dynamics are not uniform along the length of the peptide. Possible origins for the large amount of inhomogeneity present at the 49-Leucine site are discussed. (C) 2004 American Institute of Physics.