Selective activation of G‐protein subtypes by vertebrate and invertebrate rhodopsins
Selective activation of G‐protein subtypes by vertebrate and invertebrate rhodopsins
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脊椎动物和无脊椎动物视紫红质选择性激活 G 蛋白亚型
DOI：
10.1016/s0014-5793(98)01340-4
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发表时间：
1998
期刊：
影响因子：
3.5
通讯作者：
Y. Shichida
中科院分区：
文献类型：
--
作者：
A. Terakita;T. Yamashita;S. Tachibanaki;Y. Shichida
我们定量研究了牛和鱿鱼视紫红质激活G蛋白亚型的特异性，以检查每个感光细胞中的光转导级联是否由大量G蛋白亚型（Gt或Gq）的共定位决定。在不同的Gt和Gq的有效激活，牛和鱿鱼视紫红质几乎没有激活G-蛋白对应。Gt和Gq的α-和βγ-亚基的交换表明α-亚基在与各自的视紫红质的特异性结合中起关键作用。因此，G-蛋白亚型的特异性识别的每个视紫红质是一个主要的机制，在确定的光转导级联。
We have quantitatively investigated specificities in activating G-protein subtype by bovine and squid rhodopsins to examine whether or not the phototransduction cascade in each of the photoreceptor cells is determined by the colocalization of a large amount of G-protein subtype (Gt or Gq). In contrast to the efficient activation of respective Gt and Gq, bovine and squid rhodopsins scarcely activated G-protein counterparts. Exchange of α- and βγ-subunits of Gt and Gq indicated the critical role of the α-subunit in specific binding to respective rhodopsins. Thus the specific recognition of G-protein subtype by each rhodopsin is a major mechanism in determining the phototransduction cascade.
DOI：
10.1042/bj2830001
发表时间：
1992-04
期刊：
The Biochemical journal
影响因子：
--
作者：
T. Savarese;Claire-M. FRASERt
通讯作者：
T. Savarese;Claire-M. FRASERt