T-cell modulation of the antibody response to bacterial polysaccharide antigens.
T-cell modulation of the antibody response to bacterial polysaccharide antigens.
复制标题
T 细胞调节抗体对细菌多糖抗原的反应。
DOI：
10.1128/iai.57.1.180-185.1989
复制
发表时间：
1989
影响因子：
3.1
通讯作者：
Bright,R
中科院分区：
文献类型：
--
作者：
Taylor,CE;Bright,R
用亚免疫剂量的脑膜炎球菌多糖 (MP)、铜绿假单胞菌脂多糖 (PA) 或变形链球菌多糖 (SM) 预处理小鼠，会抑制抗体反应。在用 MP 免疫时，将推定的抑制性 T 细胞（Ts 细胞）从用亚免疫原性剂量的 MP 引发的供体小鼠转移到首次接受者体内，大大降低了抗体反应的强度。此外，相对于对照组，输注取自 MP 或 PA 免疫动物的 B 细胞分别抑制了首次接受者对 MP 或 PA 的抗体反应，这表明 T 细胞对免疫 B 细胞上的决定簇有反应。我们观察到，免疫后 2 天注射伴刀豆球蛋白 A 或植物血凝素（两种已知可增强扩增 T 细胞 [Ta 细胞] 活性的凝集素）可增强对 MP 和 SM 的抗体反应。此外，利用脾细胞将 Ta 细胞活性转移到初始动物体内。尽管在用MP免疫时施用植物血凝素也导致抗体反应增加，但在免疫的同时注射伴刀豆球蛋白A导致对MP的抗体反应抑制。尽管发现响应III型肺炎球菌多糖而产生的Ts细胞对单克隆抗体Ly-m22有反应，但Ta细胞对单克隆抗体L3T4和Ia有反应，但对Ly-m22没有反应。这些研究表明，Ta 和 Ts 细胞可以分别以正向和负向方式调节对 MP、SM 和 PA 的抗体反应。
Pretreatment of mice with subimmunogenic doses of meningococcal polysaccharide (MP), Pseudomonas aeruginosa lipopolysaccharide (PA), or Streptococcus mutans polysaccharide (SM) resulted in suppression of antibody response. The transfer of putative suppressor T cells (Ts cells) from donor mice primed with a subimmunogenic dose of MP to naive recipients at the time of immunization with MP substantially reduced the magnitude of the antibody response. Also, the infusion of B cells taken from animals immunized with either MP or PA suppressed the antibody response of naive recipients to MP or PA, respectively, relative to controls, suggesting that Ts cells respond to determinants on immune B cells. We observed that the injection of concanavalin A or phytohemagglutinin (two lectins known to augment the activity of amplifier T cells [Ta cells]) 2 days postimmunization enhanced the antibody response to MP and SM. In addition, Ta-cell activity was transferred to naive animals by using spleen cells. Although the administration of phytohemagglutinin at the time of immunization with MP also resulted in increased antibody response, the injection of concanavalin A simultaneous with immunization resulted in a suppression of the antibody response to MP. Although Ts cells generated in response to pneumococcal polysaccharide type III were found to respond to monoclonal antibody Ly-m22, Ta cells responded to monoclonal antibodies L3T4 and Ia but not to Ly-m22. These studies suggest that Ta and Ts cells can modulate the antibody response to MP, SM, and PA in a positive and negative manner, respectively.