VIP plus interneurons control neocortical activity across brain states
VIP plus interneurons control neocortical activity across brain states
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DOI：
10.1152/jn.01124.2015
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发表时间：
2016-06-01
影响因子：
2.5
通讯作者：
Yuste, Rafael
中科院分区：
文献类型：
--
作者：
Jackson, Jesse;Ayzenshtat, Inbal;Yuste, Rafael
GABA能中间神经元被定位为强有力地影响神经活动的动力学，然而控制自发和诱发的新皮质活动的神经元间介导的电路机制仍然难以捉摸。血管活性肠肽(VIP+)中间神经元是一类特殊的细胞，它特异性地突触于其他中间神经元，可能有助于促进皮质活动的增加。在这项研究中，我们利用体内的钙离子成像，描述了局部网络活动和VIP+细胞之间的相互作用，并确定了它们在调节小鼠视皮层新皮质活动中的作用。VIP+细胞在包括运动、非运动、视觉刺激和麻醉的大脑状态下都是活跃的。在所有大脑状态下，VIP+活性与附近兴奋性神经元群体活动的平均水平相关性最明显，这表明VIP+细胞使大脑皮层处于高兴奋性状态。对VIP+细胞输出的药物遗传阻断降低了运动、非运动、麻醉和视觉刺激时的网络活动，表明VIP+细胞在大脑皮层中对神经活动起状态独立的促进作用。总而言之，我们的发现表明，VIP+神经元在自发和刺激诱发的新皮质活动中产生高活动区域具有因果作用。
GABAergic interneurons are positioned to powerfully influence the dynamics of neural activity, yet the interneuron-mediated circuit mechanisms that control spontaneous and evoked neocortical activity remains elusive. Vasoactive intestinal peptide (VIP+) interneurons are a specialized cell class which synapse specifically on other interneurons, potentially serving to facilitate increases in cortical activity. In this study, using in vivo Ca2+ imaging, we describe the interaction between local network activity and VIP+ cells and determine their role in modulating neocortical activity in mouse visual cortex. VIP+ cells were active across brain states including locomotion, nonlocom.otion, visual stimulation, and under anesthesia. VI.P+ activity correlated most clearly with the mean level of population activity of nearby excitatory neurons during all brain states, suggesting VIP+ cells enable high-excitability states in the cortex. The pharmacogenetic blockade of VIP+ cell output reduced network activity during locomotion, nonlocomotion, anesthesia, and visual stimulation, suggesting VIP+ cells exert a state-independent facilitation of neural activity in the cortex. Collectively, our findings demonstrate that VIP+ neurons have a causal role in the generation of high-activity regimes during spontaneous and stimulus evoked neocortical activity.