Conformational behavior of giant DNA through binding with Ag+ and metallization.
Conformational behavior of giant DNA through binding with Ag+ and metallization.
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DOI：
10.1021/bm800235j
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发表时间：
2008-06
影响因子：
6.2
通讯作者：
A. Zinchenko;D. Baigl;N. Chen;O. Pyshkina;K. Endo;V. Sergeyev;K. Yoshikawa
中科院分区：
文献类型：
--
作者：
A. Zinchenko;D. Baigl;N. Chen;O. Pyshkina;K. Endo;V. Sergeyev;K. Yoshikawa
用荧光显微镜、电子显微镜和紫外光谱研究了长单链双链DNA在游离银离子和NaBH 4还原AgNO3生成的银纳米粒子溶液中的构象行为。单价银离子与DNA的相互作用通过Ag+与DNA碱基的络合作用导致DNA螺旋聚合物链的收缩，这是DNA持续长度减少的结果。相反，在DNA溶液中NaBH 4还原银离子会触发DNA压缩：DNA从伸长的卷曲状态转变为紧凑状态。这种转变是连续的，不像通常在多阳离子中看到的全或无离散DNA压缩。这表明DNA的塌陷伴随着DNA模板上生成的银纳米颗粒的生长聚集。
The conformational behavior of a long single-chain double-stranded DNA in solutions of free silver ions and silver nanoparticles generated via the reduction of AgNO3 by NaBH4 was monitored by fluorescence and electron microscopies and UV spectroscopy. The interaction of monovalent silver ions with DNA induces shrinking of a DNA-coiled polymer chain as a result of a decrease in the DNA persistence length through the complexation of Ag+ with DNA bases. In contrast, the reduction of silver ions by NaBH4 in DNA solutions triggers DNA compaction: a DNA transition from elongated coil state into a compact state. This transition is continuous, unlike the all-or-none discrete DNA compaction that is commonly seen with multications. It is suggested that the collapse of DNA is accompanied by growth aggregation of silver nanoparticles generated on the DNA template.