Structure of the RNA-Binding Domain of Nodamura Virus Protein B2, a Suppressor of RNA Interference
Structure of the RNA-Binding Domain of Nodamura Virus Protein B2, a Suppressor of RNA Interference
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DOI：
10.1021/bi900126s
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发表时间：
2009-03-24
期刊：
影响因子：
2.9
通讯作者：
Chen, Julian C. -H.
中科院分区：
文献类型：
--
作者：
Koerber, Stephanie;Ali, P. Shaik Syed;Chen, Julian C. -H.
来自Nodamura病毒（NMV B2）的蛋白B2是Nodavirus家族的成员，其作为RNA干扰（RNAi）的抑制因子。NMV B2的N-末端结构域由残基1-79组成，识别双链RNA（dsRNA）。NMV B2的RNA结合结构域的2.5埃晶体结构显示二聚体的螺旋束结构。尽管有限的序列同一性，但与鸡舍病毒B2相比，该结构显示一组保守的RNA结合残基。晶体堆积将RNA结合残基沿着一个面放置与双链RNA相关的分子，这表明了一个潜在的识别双链RNA的平台。
Protein B2 from Nodamura virus (NMV B2), a member of the Nodavirus family, acts as a suppressor of RNA interference (RNAi). The N-terminal domain of NMV B2, consisting of residues 1-79, recognizes double-stranded RNA (dsRNA). The 2.5 angstrom crystal structure of the RNA-binding domain of NMV B2 shows a dimeric, helical bundle Structure, The structure shows a conserved set of RNA-binding residues compared with flock house virus B2, despite limited sequence identity. The crystal packing places the RNA-binding residues along one face of symmetry-related molecules, suggesting a potential platform for recognition of dsRNA.