Combining plasmon-enhanced fluorescence with Rayleigh surface acoustic waves to quantify Carcinoembryonic Antigen from human plasma
Combining plasmon-enhanced fluorescence with Rayleigh surface acoustic waves to quantify Carcinoembryonic Antigen from human plasma
复制标题
结合等离子体增强荧光与瑞利表面声波来量化人血浆中的癌胚抗原
DOI：
10.1016/j.bios.2022.114822
复制
发表时间：
2023
影响因子：
12.6
通讯作者：
Bhethanabotla, Venkat
中科院分区：
文献类型：
--
作者：
Huang, Yuqi;Li, Shuangming;Bhethanabotla, Venkat
为了提高免疫荧光法直接定量检测体液中癌胚抗原（CEA）的准确性，研制了一种声表面波（SAW）生物传感器，并在传感区引入了优化的银纳米结构。通过使用快速热退火（RTA）方法图案化银纳米结构来实现荧光信号放大。此外，背景噪声干扰的问题，从非特异性结合在人血浆中的瑞利波流在免疫测定区域，这表明在检测限的降低。结果表明，银纳米结构显著地将传感器灵敏度提高了49.99倍，并且将磷酸盐缓冲盐水（PBS）溶液中的CEA的检测限降低到101.94 pg/mL。通过使用Rayleigh SAW流降低背景噪声，成功地将人血浆中CEA生物标志物的检测限降至11.81 ng/mL。这允许在各种临床生物感测应用中实现需求点传感器系统。
To improve the direct quantification of Carcinoembryonic Antigen (CEA) from body fluids by immunofluorescence, a surface acoustic wave (SAW) based biosensor was developed combined with an optimized silver nanostructure at the sensing region. Fluorescence signal amplification is achieved by patterning silver nanostructures using the rapid thermal annealing (RTA) method. In addition, the problem of background noise interference from nonspecific binding in human plasma is addressed by Rayleigh wave streaming at the immunoassay region, which shows a reduction in the limit of detection. The results show that the silver nanostructures significantly increase the sensor sensitivity by 49.99-fold and lower the limit of detection of CEA in phosphate buffered saline (PBS) solution to 101.94 pg/mL. The limit of detection of CEA biomarker in human plasma was successfully brought down to 11.81 ng/mL by reducing background noise using Rayleigh SAW streaming. This allows for a point-of-need sensor system to be realized in various clinical biosensing applications.