Backbone and side chain NMR assignments and secondary structure calculation of the pheromone binding protein3 of Ostrinia nubilalis, an agricultural pest
Backbone and side chain NMR assignments and secondary structure calculation of the pheromone binding protein3 of Ostrinia nubilalis, an agricultural pest
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DOI：
10.1007/s12104-023-10145-3
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发表时间：
2023-07-27
影响因子：
0.9
通讯作者：
Mohanty，Smita
中科院分区：
文献类型：
--
作者：
Al-Danoon，Omar;Mohanty，Smita
玉米螟（Ostrinia nubilalis），又称欧洲玉米螟（ECB），是欧洲和北美以及中亚和北非的严重害虫。它危害多种农作物，如玉米、燕麦、荞麦、小米和大豆。每年造成至少10亿美元的损失。在雄蛾触角中优先表达的ostrinia nubilalispheromone-binding protein3 （OnubPBP3）参与了交配过程中雌蛾分泌的信息素混合物的检测。了解OnubPBP3的结构和功能，包括信息素结合及其在树突嗅觉神经元（ORN）释放的机制，对于通过感觉抑制实现害虫综合治理至关重要。在这里，我们报告了OnubPBP3在pH 6.5下的主链和侧链共振分配，通过各种三重共振核磁共振实验在a13C， 15n标记的蛋白质样品上进行。OnubPBP3的二级结构由6个α-螺旋和一个基于主链化学位移的非结构化c端组成。
Ostrinia nubilalis, also known as European Corn Borer (ECB), is a serious pest in Europe and North America, as well as in Central Asia and Northern Africa. It damages a variety of agricultural crops such as corn, oats, buckwheat, millet, and soybeans. causing annually at least one billion dollars in loss. TheOstrinia nubilalispheromone-binding protein3 (OnubPBP3), preferentially expressed in the male moth antenna, has been implicated in the detection of the female-secreted pheromone blend during the mating process. Understanding the structure of and function of OnubPBP3, including the mechanism of pheromone binding and its release at the dendritic olfactory neuron (ORN), is essential if integrated pest management through sensory inhibition is to be achieved. We report here the backbone and side-chain resonance assignments of OnubPBP3 at pH 6.5 using various triple resonance NMR experiments on a13C,15N-labeled protein sample. The secondary structure of OnubPBP3 consists of six α-helices and an unstructured C-terminus based on backbone chemical shifts.