Coccidioides immitis isolated from armadillos (Dasypus novemcinctus) in the state of Piaui, northeast Brazil
Coccidioides immitis isolated from armadillos (Dasypus novemcinctus) in the state of Piaui, northeast Brazil
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DOI：
10.1023/a:1007273019647
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发表时间：
2001-01-01
期刊：
影响因子：
5.5
通讯作者：
Wanke, B
中科院分区：
文献类型：
--
作者：
Eulálio, KD;de Macedo, RL;Wanke, B
在球孢子菌病流行的巴西东北部皮奥伊州研究了犰狳自然感染粗球孢子菌的情况。1998年，在4个不同的县捕获了26只九带犰狳（Dasypus novemcinctus）。在乙醚深度麻醉下处死动物。在尸检时，将脾、肝、肺和心脏的碎片均质化，并接种到含有和不含放线菌酮的沙氏葡萄糖琼脂（BBL，USA）上。每个器官的一部分也被处理用于组织学检查。将在室温下孵育第二周后观察到的丝状真菌的疑似菌落（显示出与空的空间交替的桶形节孢子）腹膜内接种到小鼠中。3只犰狳被证实感染了C.免疫系统。用从三只犰狳脾匀浆和两只犰狳肝匀浆中获得的可疑菌落接种小鼠，发生了播散性球孢子菌病和未成熟和成熟的球孢子菌。在几个器官中发现了免疫炎。第一次发现犰狳（D. novemcinctus）自然感染C. immitis，增加了关于生态学的新数据，以及这些祖先哺乳动物在这种真菌进化生命周期中可能发挥的作用。
Natural infection of armadillos with Coccidioides immitis was studied in the state of Piaui, northeast of Brazil, endemic for coccidioidomycosis. In 1998, 26 nine-banded armadillos (Dasypus novemcinctus) were captured in 4 different counties. The animals were sacrificed under deep anesthesia with ether. At necropsy fragments of spleen, liver, lungs and heart were homogenized and seeded onto Sabouraud dextrose agar with and without cycloheximide (BBL, USA). Part of each organ was also processed for histological examination. Suspected colonies of filamentous fungi observed after the second week of incubation at room temperature, exhibiting barrel-shaped arthroconidia alternating with empty spaces, were inoculated intraperitoneally into mice. Three armadillos proved to be infected with C. immitis. Mice inoculated with suspected colonies obtained from homogenized spleen of three and liver of two armadillos developed disseminated coccidioidomycosis and immature and mature spherules of C. immitis were disclosed in several organs. For the first time armadillos (D. novemcinctus) were found naturally infected with C. immitis, adding new data on the ecology and on a possible role of these ancestral mammals in the evolutionary life cycle of this fungus.