Electron Cryotomography of Tula Hantavirus Suggests a Unique Assembly Paradigm for Enveloped Viruses
Electron Cryotomography of Tula Hantavirus Suggests a Unique Assembly Paradigm for Enveloped Viruses
复制标题
DOI：
10.1128/jvi.00057-10
复制
发表时间：
2010-05-01
影响因子：
5.4
通讯作者：
Gruenewald, Kay
中科院分区：
文献类型：
--
作者：
Huiskonen, Juha T.;Hepojoki, Jussi;Gruenewald, Kay
汉坦病毒(布尼亚病毒科)是由啮齿动物传播的新出现的病毒，对人类健康造成严重的世界性威胁。汉坦病毒病包括肾综合征出血热和汉坦病毒心肺综合征。病毒粒子被包被，并含有一个三部分单链负义RNA基因组。两种类型的糖蛋白，G(N)和G(C)，嵌入在病毒的膜上，形成突起或“尖峰”。膜包裹着一个核糖核蛋白核心，它由RNA片段、核衣壳蛋白和依赖RNA的RNA聚合酶组成。关于汉坦病毒病毒粒子结构和糖蛋白尖峰组成的详细信息很少。在这里，我们用电子冷冻显微镜和断层扫描技术研究了图拉汉坦病毒粒子的结构。三维密度图显示了汉坦病毒表面糖蛋白、膜和核糖核蛋白是如何组织的。通过平均层析子体积，G(N)-G(C)尖峰复合体的结构被解析到3.6 nm分辨率。每个扣球复合体都是具有4重对称性的正方形组件。刺突复合体通过特定的侧向相互作用在病毒膜上形成有序的斑块。这些相互作用可能足以在病毒萌发期间产生膜曲率。总之，图拉汉坦病毒的结构和组装原理体现了一种独特的包膜病毒组装范例。
Hantaviruses (family Bunyaviridae) are rodent-borne emerging viruses that cause a serious, worldwide threat to human health. Hantavirus diseases include hemorrhagic fever with renal syndrome and hantavirus cardiopulmonary syndrome. Virions are enveloped and contain a tripartite single-stranded negative-sense RNA genome. Two types of glycoproteins, G(N) and G(C), are embedded in the viral membrane and form protrusions, or "spikes." The membrane encloses a ribonucleoprotein core, which consists of the RNA segments, the nucleocapsid protein, and the RNA-dependent RNA polymerase. Detailed information on hantavirus virion structure and glycoprotein spike composition is scarce. Here, we have studied the structures of Tula hantavirus virions using electron cryomicroscopy and tomography. Three-dimensional density maps show how the hantavirus surface glycoproteins, membrane, and ribonucleoprotein are organized. The structure of the G(N)-G(C) spike complex was solved to 3.6-nm resolution by averaging tomographic subvolumes. Each spike complex is a square-shaped assembly with 4-fold symmetry. Spike complexes formed ordered patches on the viral membrane by means of specific lateral interactions. These interactions may be sufficient for creating membrane curvature during virus budding. In conclusion, the structure and assembly principles of Tula hantavirus exemplify a unique assembly paradigm for enveloped viruses.