Monoclonal radioimmunoassays for hepatitis B surface antigen: demonstration of hepatitis B virus DNA or related sequences in serum and viral epitopes in immune complexes.
Monoclonal radioimmunoassays for hepatitis B surface antigen: demonstration of hepatitis B virus DNA or related sequences in serum and viral epitopes in immune complexes.
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乙型肝炎表面抗原的单克隆放射免疫测定：证明血清中的乙型肝炎病毒 DNA 或相关序列以及免疫复合物中的病毒表位。
DOI：
10.1073/pnas.79.18.5675
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发表时间：
1982
影响因子：
11.1
通讯作者：
Wands,JR
中科院分区：
文献类型：
--
作者：
Shafritz,DA;Lieberman,HM;Isselbacher,KJ;Wands,JR
我们最近研制了抗B型肝炎表面抗原（HBsAg）的高亲和力单克隆IgM和IgG抗体（抗-HBs），并用于建立高灵敏度的放射免疫分析法，以检测血清中HBsAg相关决定簇。我们现在报告，通过分子杂交分析，证明单克隆免疫反应性物质的选定血清样本也含有与B型肝炎病毒（HBV）DNA互补的核酸序列。此外，单克隆放射免疫测定法可以检测在抗-HBs过量时形成的HBsAg-抗-HBs免疫复合物中的病毒决定簇;这些决定簇是商业放射免疫测定法检测不到的。这些和我们以前的研究表明，有HBV或“HBV相关”的代理人存在于人血清中，可检测到的单克隆抗体，但不能识别的常规多价抗体。
High-affinity monoclonal IgM and IgG antibodies (anti-HBs) to hepatitis B surface antigen (HBsAg) have recently been developed and used by us in the construction of highly sensitive radioimmunoassays for the detection of HBsAg-associated determinants in serum. We now report that selected serum samples demonstrating monoclonal immunoreactive material also contain nucleic acid sequences complementary to hepatitis B virus (HBV) DNA by molecular hybridization analysis. In addition, monoclonal radioimmunoassays can detect viral determinants in HBsAg-anti-HBs immune complexes formed in anti-HBs excess; such determinants are undetectable by commercial radioimmunoassay. These and our previous studies suggest that there are HBV or "HBV-related" agents present in human serum that are detected by monoclonal antibodies but are not identified by conventional polyvalent antibodies.