A MONOCLONAL-ANTIBODY TO THE DOUBLE-STRANDED POLYRIBONUCLEOTIDE COMPLEX POLY(A).POLY(U)
A MONOCLONAL-ANTIBODY TO THE DOUBLE-STRANDED POLYRIBONUCLEOTIDE COMPLEX POLY(A).POLY(U)
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DOI：
10.1016/0161-5890(84)90150-0
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发表时间：
1984-01-01
影响因子：
3.6
通讯作者：
LACOUR, F
中科院分区：
文献类型：
--
作者：
DELAGE, G;NAHON, E;LACOUR, F
双链多核糖核苷酸复合物poly(A).cntdot.poly(U)的单克隆抗体源自免疫DBA/2小鼠的脾细胞与P3.cntdot.X63-Ag8浆细胞瘤的融合。使用放射免疫测定的特异性研究表明，抗-poly(A).cntdot.poly(U) 不会与单链多核糖核苷酸、RNA.cntdot.DNA 杂交体或 DNA 发生交叉反应。除了关联腺嘌呤和尿嘧啶的 RNA 双链体之外，它还能识别合成的 poly(I).cntdot.poly(C) 和天然存在的呼肠孤病毒 RNA。因此，它针对绝对需要 2 条多核糖磷酸链的构象表位。该抗体不会与 poly(G).cntdot.poly(C) 发生交叉反应，因此能够区分 RNA 双螺旋。
A monoclonal antibody to the double-stranded polyribonucleotide complex poly(A).cntdot.poly(U) was derived from the fusion of spleen cells from immunized DBA/2 mice and the P3.cntdot.X63-Ag8 plasma cytoma. Specificity studies using radioimmunoassays showed that the anti-poly(A).cntdot.poly(U) does not cross-react with single-stranded polyribonucleotides, RNA.cntdot.DNA hybrids or DNA. In addition to RNA duplexes associating adenine and uracil, it recognizes synthetic poly(I).cntdot.poly(C) and naturally occurring reovirus RNA. It is thus directed against a conformational epitope with an absolute requirement for 2 polyribose phosphate chains. The antibody does not cross-react with poly(G).cntdot.poly(C) and is therefore able to distinguish between RNA double helices.