Characterization of an antimicrobial material from a newly isolated Bacillus amyloliquefaciens from mangrove for biocontrol of Capsicum bacterial wilt
Characterization of an antimicrobial material from a newly isolated Bacillus amyloliquefaciens from mangrove for biocontrol of Capsicum bacterial wilt
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DOI：
10.1016/j.biocontrol.2010.06.015
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发表时间：
2010-09-01
影响因子：
4.2
通讯作者：
He, Hong
中科院分区：
文献类型：
--
作者：
Hu, Han Qiao;Li, Xin Shen;He, Hong
了解植物内生拮抗细菌的作用机制有助于植物病害的防治。从红树林中分离到一株内生细菌Bg-C31，经盆栽和田间试验表明，Bg-C31对辣椒青枯病具有明显的生防作用。通过生理生化试验、16 S rDNA和LCI基因序列分析，确定Bg-C31为解淀粉芽孢杆菌（Bacillus amyloliquefaciens）。通过硫酸铵沉淀和丁醇萃取，Bg-C31产生的抗菌物质被鉴定为一种蛋白质，其对蛋白酶k和热具有抗性。利用质粒消除法将拮抗基因定位在染色体上。表达了LCI基因的29 kDa融合蛋白。在凝胶原位上检测到融合蛋白对青枯菌的抗菌活性，表明LCI基因可能用于产生抗细菌感染的转基因植物。(C)2010年由Elsevier Inc.出版
Understanding the mechanisms of the antagonistic endophytic bacteria is helpful in controlling plant diseases. An endophytic bacterium, Bg-C31, from mangrove was found to be antagonistic to some fungal and bacterial pathogens of plants and to be effective in the biocontrol of Capsicum bacterial wilt in pot and field trials. Bg-C31 was identified as Bacillus amyloliquefaciens by biochemical and physiological tests as well as sequences of 16S rDNA and the LCI gene. The antimicrobial substance produced by Bg-C31 was identified as a protein, which is resistant to protease k and heat, by ammonium sulfate precipitation and butanol extraction. The antagonistic gene was located in the chromosome by plasmid curing. A 29 kDa fusion protein of the LCI gene was expressed. Antimicrobial activity of the fusion protein to Ralstonia solanacearum was detected on gels in situ, indicating that the LCI gene could potentially be used to produce transgenic plants that are resistant to bacterial infection. (C) 2010 Published by Elsevier Inc.