A novel sex difference in Drosophila contact chemosensory neurons unveiled using single cell labeling
A novel sex difference in Drosophila contact chemosensory neurons unveiled using single cell labeling
复制标题
DOI：
10.1080/01677063.2018.1531858
复制
发表时间：
2019-04-03
影响因子：
1.9
通讯作者：
Yamamoto, Daisuke
中科院分区：
文献类型：
--
作者：
Kimura, Ken-ichi;Urushizaki, Akira;Yamamoto, Daisuke
在控制果蝇交配行为的感觉方式中，接触性信息素起着主要作用。接触性信息素的关键受体神经元位于前肢上，在求偶动作中的轻拍事件期间，当雄性触摸雌性腹部时，前肢被激活。一个无结果的（fru）阳性（fru [+]）雄性信息素感受细胞（M-细胞）和一个fru [+]雌性信息素感受细胞（F-细胞）在腿上的感觉刚毛中配对，一些fru [+]化学感受器轴突在雄性而不是雌性的胸神经节中穿过中线投射。然而，在胸神经节中形成性二态轴突终末的受体细胞仍然未知。通过产生标记的单细胞克隆，我们发现只有特定的fru [+]化学感受神经元亚群具有跨越男性中线的轴突。我们进一步证明，存在两个男性特有的刚毛，每个窝藏两个化学感觉神经元，其中没有表现出中线交叉，阳性特征。这项研究揭示了迄今尚未认识到的化学感受神经元的性别差异，迫使我们重新调查信息素输入途径，冲击中央求偶电路。
Among the sensory modalities involved in controlling mating behavior in Drosophila melanogaster, contact sex pheromones play a primary role. The key receptor neurons for contact sex pheromones are located on the forelegs, which are activated in males upon touching the female abdomen during tapping events in courtship actions. A fruitless (fru)-positive (fru [+]) male-pheromone sensing cell (M-cell) and a fru [+] female-pheromone sensing cell (F-cell) are paired in a sensory bristle on the legs, and some fru [+] chemoreceptor axons project across the midline in the thoracic neuromere in males but not in females. However, the receptor cells that form sexually dimorphic axon terminals in the thoracic ganglia remain unknown. By generating labeled single-cell clones, we show that only a specific subset of fru [+] chemosensory neurons have axons that cross the midline in males. We further demonstrate that there exist two male-specific bristles, each harboring two chemosensory neurons; neither of which exhibits midline crossing, a masculine characteristic. This study reveals hitherto unrecognized sex differences in chemosensory neurons, imposing us to reinvestigate the pheromone input pathways that impinge on the central courtship circuit.