Mesophilic anaerobic biodegradation test and analysis of eubacteria and archaea involved in anaerobic biodegradation of four specified biodegradable polyesters
Mesophilic anaerobic biodegradation test and analysis of eubacteria and archaea involved in anaerobic biodegradation of four specified biodegradable polyesters
复制标题
四种特定可生物降解聚酯厌氧生物降解涉及的真细菌和古细菌的中温厌氧生物降解试验和分析
DOI：
10.1016/j.polymdegradstab.2014.08.031
复制
发表时间：
2014
影响因子：
5.9
通讯作者：
Masao Kunioka
中科院分区：
文献类型：
--
作者：
Hisaaki Yagi;Fumi Ninomiya;Masahiro Funabashi;Masao Kunioka
使用我们先前报告的评估系统（修改的ISO 13975方法），在37 °C下在水生条件下比较了四种生物塑料粉末（125-250 μm），聚己内酯（PCL），聚乳酸（PLA），聚羟基丁酸酯（PHB）和聚丁二酸丁二醇酯（PBS）的厌氧生物降解性。生物塑料的厌氧生物降解率为PHB >> PLA > PCL。聚羟基丁酸酯、聚乳酸和聚己内酯的生物降解率分别为90%，29%和49%，277天，3%和22%，在9天。PBS不能被本研究中使用的污泥厌氧生物降解。采用RT-PCR DGGE方法检测了参与厌氧生物塑料降解的真细菌和古细菌。参与PCL和PLA厌氧生物降解的真细菌有其独特性。参与厌氧降解PHB的真细菌分别与Arcostereius、Clostridium sp和Clostridium sp有98%的同源性。参与PLA厌氧降解的真细菌与黄单胞菌科细菌和中生根瘤菌属细菌的同源性为100%;然而，其他三种细菌与任何培养细菌或数据库序列的相关性都很差（<95%相似性）。PCL的厌氧生物降解由真细菌进行，与A thereius的相似性为98%，而由5种生物进行，与任何培养细菌或数据库序列的相似性为低（≤95%）。参与PLA和PCL厌氧生物降解的古菌与Methanosaetaconciliii和Methanobacteriumpetrolearium的同源性分别为97%和95%，而参与PHB厌氧生物降解的古菌的RT-PCR DGGE图谱只有微弱的条带。
The anaerobic biodegradability of four bioplastics powders (125–250 μm), polycaprolactone (PCL), poly(lactic acid) (PLA), polyhydroxybutyrate (PHB) and poly(butylene succinate) (PBS), were compared at 37 °C under aquatic conditions using our previously reported evaluation system (the modified ISO 13975 method). The bioplastics were ranked PHB >> PLA > PCL in terms of anaerobic biodegradation rate. The biodegradability of PHB, PLA, and PCL was 90% in 9 days, 29% and 49% in 277 days, and 3% and 22% in 277 days, respectively. PBS could not be anaerobically biodegraded by the sludge used in this study. The eubacteria and archaea participating in anaerobic bioplastic biodegradation were detected using the RT-PCR DGGE method. Some eubacteria participating in anaerobic biodegradation of PCL and PLA were of unique character. Eubacteria involved in anaerobic biodegradation of PHB were 98% homologous with Arcobacterthereius, Clostridium sp, andClostridiumsp, respectively. The eubacteria involved in the anaerobic biodegradation of PLA were 100% identical to Xanthomonadaceae bacterium and Mesorhizobium sp.; however, the other three bacterium were poorly related (<95% similarity) to any of the cultured-bacterium or database sequences. Anaerobic biodegradation of PCL was conducted by eubacterium with 98% similarity toA thereius, and by five organisms with poor similarity (≤95%) to any of the cultured-bacterium or database sequences. Archaea involved in anaerobic biodegradation of PLA and PCL were 97% and 95% identical toMethanosaetaconciliiand Methanobacteriumpetrolearium.Only faint bands were observed in the RT-PCR DGGE profile of the archaea generated during PHB anaerobic biodegradation.