Phosphate Transporter PvPht1;2 Enhances Phosphorus Accumulation and Plant Growth without Impacting Arsenic Uptake in Plants
Phosphate Transporter PvPht1;2 Enhances Phosphorus Accumulation and Plant Growth without Impacting Arsenic Uptake in Plants
复制标题
磷酸盐转运蛋白 PvPht1;2 增强磷积累和植物生长，而不影响植物对砷的吸收
DOI：
10.1021/acs.est.7b06674
复制
发表时间：
2018-04-03
影响因子：
11.4
通讯作者：
Ma, Lena Q.
中科院分区：
文献类型：
--
作者：
Cao, Yue;Sun, Dan;Ma, Lena Q.
磷是植物生长所需的重要常量营养元素，植物主要以磷(P)的形式获得磷。磷酸盐转运体（Phts）负责植物对磷和砷酸盐（AsV）的吸收，包括砷-超富集植物Pteris vittata。vittata对AsV的吸收和磷的利用是有效的，但其吸收磷的分子机制在很大程度上是未知的。在本研究中，一个P. vittata Pht, PvPht1；2 .被克隆并转化为烟草（Nicotiana tabacum）。在水培试验中，所有转基因系的P含量均显著高于野生型，且生长较野生型好，说明PvPht1；2介导的植物磷吸收。此外，表达PvPht1；2也使茎/根P-32比值提高了69 ~ 92%，使木质部液P提高了46 ~ 62%，表明PvPht1；2也介导植物磷转运。与许多可渗透AsV的pht不同，PvPht1；2株无AsV转运能力。土壤试验中，PvPht1；2在不增加PvPht1 As积累的情况下显著增加了地上部生物量；2 .转基因烟草。综上所述，我们的结果表明PvPht1；2是一种特殊的磷转运蛋白，负责植物磷的获取和转运。我们设想PvPht1；2可以在不影响AsV吸收的情况下提高作物P的获取，从而在不影响食品安全的情况下提高作物产量。
Phosphorus is an important macronutrient for plant growth and is acquired by plants mainly as phosphate (P). Phosphate transporters (Phts) are responsible for P and arsenate (AsV) uptake in plants including arsenic-hyperaccumulator Pteris vittata. P. vittata is efficient in AsV uptake and P utilization, but the molecular mechanism of its P uptake is largely unknown. In this study, a P. vittata Pht, PvPht1;2, was cloned and transformed into tobacco (Nicotiana tabacum). In hydroponic experiments, all transgenic lines displayed markedly higher P content and better growth than wild type, suggesting that PvPht1;2 mediated P uptake in plants. In addition, expressing PvPht1;2 also increased the shoot/root P-32 ratio by 69-92% and enhanced xylem sap P by 46-62%, indicating that PvPht1;2 also mediated P translocation in plants. Unlike many Phts permeable to AsV, PvPht1;2 showed little ability to transport AsV. In soil experiments, PvPht1;2 also significantly increased shoot biomass without elevating As accumulation in PvPht1;2 transgenic tobacco. Taken together, our results demonstrated that PvPht1;2 is a specific P transporter responsible for P acquisition and translocation in plants. We envisioned that PvPht1;2 can enhance crop P acquisition without impacting AsV uptake, thereby increasing crop production without compromising food safety.