Single molecule mtDNA fiber FISH for analyzing numtogenesis.
Single molecule mtDNA fiber FISH for analyzing numtogenesis.
复制标题
用于分析神经发生的单分子 mtDNA 纤维 FISH。
DOI：
10.1016/j.ab.2017.03.015
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发表时间：
2018
影响因子：
2.9
通讯作者：
Singh,KeshavK
中科院分区：
文献类型：
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作者：
Koo,Dal-Hoe;Singh,Bhupendra;Jiang,Jiming;Friebe,Bernd;Gill,BikarmS;Chastain,PaulD;Manne,Upender;Tiwari,HemantK;Singh,KeshavK
人体体细胞含有数千个线粒体DNA （mtDNA）拷贝。在真核生物中，mtDNA自然转移到细胞核中产生核线粒体DNA （NUMT）拷贝。我们将这种现象称为“钱币生成”。Numtogenesis是一个成熟的进化过程，报道了各种测序真核生物基因组。我们已经建立了一个快速检测和分析全基因组中NUMT插入的分子工具。迄今为止，NUMT分析依赖于深度基因组测序结合全基因组的综合计算分析。这是耗时、繁琐和成本高昂的。此外，由于技术有限，大多数实验室无法完成这种分析。我们报道了单分子mtFIBER FISH（荧光原位杂交）的发展，以研究numtogenesis。mtFIBER FISH的开发应该有助于确定细胞生成在癌症和其他人类疾病中的作用。这项新技术将有助于区分和监测癌症的分期和进展，有助于阐明肿瘤发生的基本机制，并有助于分析与癌症早期检测、筛查和/或癌症风险评估相关的过程。
Somatic human cells contain thousands of copies of mitochondrial DNA (mtDNA). In eukaryotes, natural transfer of mtDNA into the nucleus generatesnuclearmitochondrial DNA (NUMT) copies. We name this phenomenon as “numtogenesis”. Numtogenesis is a well-established evolutionary process reported in various sequenced eukaryotic genomes. We have established a molecular tool to rapidly detect and analyze NUMT insertions in whole genomes. To date, NUMT analyses depend on deep genome sequencing combined with comprehensive computational analyses of the whole genome. This is time consuming, cumbersome and cost prohibitive. Further, most laboratories cannot accomplish such analyses due to limited skills. We report the development of single-molecule mtFIBER FISH (fluorescence in situ hybridization) to study numtogenesis. The development of mtFIBER FISH should aid in establishing a role for numtogenesis in cancers and other human diseases. This novel technique should help distinguish and monitor cancer stages and progression, aid in elucidation of basic mechanisms underlying tumorigenesis and facilitate analyses of processes related to early detection of cancer, screening and/or cancer risk assessment.