Transcriptome analysis of ultraviolet A-induced photoaging cells with deep sequencing
Transcriptome analysis of ultraviolet A-induced photoaging cells with deep sequencing
复制标题
通过深度测序对紫外线 A 诱导的光老化细胞进行转录组分析
DOI：
10.1111/1346-8138.14157
复制
发表时间：
2018
影响因子：
3.1
通讯作者：
Lai Wei
中科院分区：
文献类型：
--
作者：
Zheng Yue;Xu Qingfang;Chen Haiyan;Chen Qiaoping;Gong Zijian;Lai Wei
基因表达变化与许多生物学过程相关。然而，紫外线（UV）-A辐射对皮肤光老化引起的遗传改变的相对后果仍然不清楚。在这里，我们对转录组进行了深度测序，并探索了与重复紫外线照射的人皮肤成纤维细胞（HDF）中生物学变化相关的改变基因，以更好地了解皮肤光老化机制。对重复UV‐A‐照射组（10 J/cm 2 UV ‐A诱导HDF，每日2次，持续7天）和对照组（HDF未照射）进行评价。使用Illumina HiSeq 2500平台和DEGseq上的高通量测序测量并比较表达基因谱。通过National Center for Biotechnology Information、Uniprot、Gene Ontology和京都基因和基因组百科全书数据库进行表达改变的基因的功能注释和代谢途径分析。通过定量逆转录聚合酶链反应验证皮肤光老化相关基因。转录组比较显示，607个基因表现出显著变化（P< 0.05），其中238个基因在UV-A照射的HDF中上调，369个基因下调。功能注释表明，UV-A辐射改变的基因参与了多种生物学过程、细胞组分合成、分子功能和代谢途径。编码弹性蛋白、sprout、组织蛋白酶K、组织蛋白酶D、组织蛋白酶B核糖磷酸二磷酸激酶和磷酸葡萄糖变位酶的光老化相关基因被鉴定为发生变化。我们获得了重复UV-A刺激HDF中的全面转录组和改变的基因，并确定了调节的基因与广泛的途径和功能相关。我们的研究结果提供了新的见解光老化的分子机制，并提出了一些新的目标，在皮肤光老化的干预。
Gene expression changes associate with many biological processes. However, the relative consequences of the genetic alterations induced by ultraviolet (UV)‐A radiation on skin photoaging are still not clear. Here, we performed deep sequencing of the transcriptome and explored altered genes related to biological changes in repeated UV‐A‐irradiated human dermal fibroblasts (HDF) to better understand the skin photoaging mechanisms. The repeatedly UV‐A‐irradiated group (HDF were induced by 10 J/cm2UV‐A twice daily for 7 days) and the control group (HDF without irradiation) were evaluated. Expression genes profile was measured and compared using high‐throughput sequencing on an Illumina HiSeq 2500 platform and DEGseq. Functional annotation and metabolic pathway analysis of genes with altered expression were preformed via National Center for Biotechnology Information, Uniprot, Gene Ontology and Kyoto Encyclopedia of Genes and Genomes databases. Genes related to skin photoaging were verified by quantitative reverse transcription polymerase chain reaction. Transcriptome comparison revealed that 607 genes exhibited significant changes (P< 0.05), of which 238 genes were upregulated and 369 downregulated in UV‐A‐irradiated HDF. Functional annotations showed that genes altered by UV‐A irradiation took part in a variety of biological process, cellular component synthesis, molecular function and metabolic pathway. Photoaging‐related genes encoding elastin, sprout, cathepsin K, cathepsin D, cathepsin B ribose‐phosphate diphosphokinase and phosphoglucomutase were identified to be changed. We obtained the comprehensive transcriptome and altered genes in repeated UV‐A‐irritated HDF and identified that the modulated genes were related to a wide panel of pathways and functions. Our results provide new insights into photoaging molecular mechanisms and suggest some novel targets for interfering in skin photoaging.