Application of affinity chromatography for separation and quantitation of glycosylated hemoglobins.
Application of affinity chromatography for separation and quantitation of glycosylated hemoglobins.
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亲和层析在糖基化血红蛋白分离和定量中的应用。
DOI：
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发表时间：
1983
期刊：
影响因子：
--
通讯作者：
M. Stallings
中科院分区：
文献类型：
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作者：
E. Abraham;R. E. Perry;M. Stallings
本文研究了间氨基苯硼酸固定在琼脂糖上作为亲和基质对糖血红蛋白的分离和定量。从亲和柱分离的糖组分含有约10%的Hb A1a + B、52%的Hb A1c和38%的Hb A0样糖组分。非糖组分主要由Hb A1 a + B和Hb A0组成，并含有少量Hb A1 c（还含有Hb F）。在正常人和糖尿病患者中，亲和法与离子交换色谱法、荧光法和比色法的比较显示出良好的相关性。亲和方法也显示出可接受的精密度和重现性。不稳定醛亚胺的存在不影响亲和方法，因为似乎只有稳定的酮胺结合到亲和凝胶上，从而通过该方法分离。此外，该方法对柱温和样品年龄的变化不太敏感。在新生儿和患有各种血红蛋白病的成人中测定糖化血红蛋白水平。结果表明，Hb F、Hb S和Hb C的存在不影响糖化血红蛋白的测定。因此，亲和色谱法比离子交换色谱法具有更广泛的应用，同时比化学方法简单和快速得多。
The use of m-aminophenylboronic acid immobilized on agarose as the affinity matrix for the separation and quantitation of Glyco Hb has been investigated. The glyco fraction isolated from the affinity columns contained about 10% Hb A1a + b, 52% Hb A1c, and 38% Hb A0-like glyco components. The nonglyco fraction had major portions of Hb A1a + b and Hb A0 and a small amount of Hb A1c (also contained Hb F). In normals and diabetic patients, the comparison of the affinity method with the ion-exchange chromatographic, fluorometric, and colorimetric methods showed good correlation. The affinity method also showed acceptable precision and reproducibility. The presence of labile aldimine did not influence the affinity method because it seems that only the stable ketoamine was bound to the affinity gel and thus separated by this method. Moreover, this method was less sensitive to variations in column temperature and sample age. Glyco Hb levels were determined in newborn infants and in adults with various hemoglobinopathies. The results indicated that the presence of Hb F, Hb S, and Hb C did not affect the glyco Hb determinations. Thus the affinity chromatographic approach has wider application than ion-exchange chromatography and is, at the same time, much simpler and faster than the chemical methods.
患有或不患有糖尿病的镰状细胞杂合子和纯合子中的次要血红蛋白。
DOI：
10.1016/0005-2795(80)90113-0
发表时间：
1980
期刊：
Biochimica et biophysica acta
影响因子：
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作者：
Abraham,EC;Stallings,M;Cameron,BF;Huisman,TH
通讯作者：
Huisman,TH