Salivary mutans streptococci and caries prevalence in 8-year-old Swedish schoolchildren.
Salivary mutans streptococci and caries prevalence in 8-year-old Swedish schoolchildren.
复制标题
瑞典 8 岁学童的唾液变形链球菌和龋齿患病率。
作者：
S. Twetman;N. Frostner
本研究的目的是获得有关唾液突变链球菌定量分布的横断面信息，用临床适用的微生物学方法评估，以及与幼龄儿童龋齿的关系。通过特制的“条带-突变”法，在选择性的肉汤中培养，对355名8岁儿童唾液中突变链球菌的存在和数量进行了检测。首先通过制造商提供的模板目视检查和分组，然后转移到实验室，借助体视显微镜进行菌落形成单位（CFU）的计数。从牙科记录中获得龋齿评分。在70%的儿童中发现了变形链球菌。46%的材料中没有细菌或含有低水平（0-10 CFU），而只有6%的材料中含有高水平（大于或等于500 CFU）。不同程度的变形链球菌感染与原发性（dmfs）和永久性（dmfs）牙列的龋齿经历之间存在强正相关（p < 0.001）。直接检查与显微镜计数有统计学意义（r = 0.84）。本研究所采用的细菌培养技术是实用可靠的，在小儿牙科临床常规中有一定的应用价值。
The aim of this study was to obtain cross-sectional information about the quantitative distribution of salivary mutants streptococci assessed with a clinically applicable microbiological method and the relation to dental caries in young schoolchildren. A total of 355 8-year-old children were examined for the presence and number of mutants streptococci in saliva by a specially prepared strip, the "Strip-mutans" method, cultivated in a selective broth. The strips were first visually examined and grouped by aid of a templet provided by the manufacturer and thereafter transferred to a laboratory for enumeration of colony forming units (CFU) with aid of a stereomicroscope. Caries scores were obtained from the dental records. Mutans streptococci were identified in 70% of all children. 46% were free from the bacterium or harboured low levels (0-10 CFU) while high levels (greater than or equal to 500 CFU) were found only in 6% of the material. Strong positive correlations (p less than 0.001) were found between different levels of mutans streptococci infection and the caries experience in both the primary (dmfs) and the permanent (DMFS) dentition. A statistically significant correlation between the direct examination and the microscopic enumeration was disclosed (r = 0.84). The bacterial culturing technique used in this study proved to be practical and reliable and thus useful in clinical routines of pediatric dentistry.