Antidepressant-like effect of cordyceps sinensis in the mouse tail suspension test
Antidepressant-like effect of cordyceps sinensis in the mouse tail suspension test
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DOI：
10.1248/bpb.30.1758
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发表时间：
2007-09-01
影响因子：
2
通讯作者：
Matsuoka, Masaaki
中科院分区：
文献类型：
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作者：
Nishizawa, Koji;Torii, Kosuke;Matsuoka, Masaaki
冬虫夏草(CS)是一种传统中药成分，具有抗疲劳、免疫调节、降血糖等多种生物学作用。由于疲劳与抑郁密切相关，我们利用小鼠的尾部悬吊试验(TST)来检测CS的热水提取物(HWCS)和超临界流体提取物(SCCS)的抗抑郁作用。SCCS(2.5-10ml/kg，P.O.)可缩短小鼠在TST的不动时间。HWCS(500-2000 mg/kg，P.O.)治疗不能使其降低，但呈剂量依赖关系。在旷场测试中，HWCS和SCCS都没有改变运动活动，排除了SCCS的影响是由于运动激活的可能性。预先给予肾上腺素受体拮抗剂哌唑嗪或多巴胺D2受体拮抗剂舒必利可减少SCCS对不动时间的影响。相反，用5-羟色胺合成抑制剂对氯苯丙氨酸(p-CPA)预处理并不改变SCCS的抗不动作用。最后一项发现与另一项观察结果一致，即SCCS对5-羟基-L-色氨酸诱导的小鼠头部抽动反应没有影响。综上所述，这些结果表明，SCCS可能通过影响肾上腺素能和多巴胺能系统而不是通过影响5-羟色胺能系统而产生抗抑郁药样作用。
Cordyceps sinensis (CS) has been known as a component of traditional medicines that elicit various biological effects such as anti-fatigue, immunomodulatory, and hypoglycemic actions. Since it has been well-established that fatigue is closely related to depression, we used the tail suspension test (TST) in mice to examine the antidepressant-like effects of hot water extract (HWCS) and supercritical fluid extract (SCCS) of CS. Immobility time in the TST was reduced by administration of SCCS (2.5-10 ml/kg, p.o.) dose-dependently though it was not reduced by treatment with HWCS (500-2000 mg/kg, p.o.). Neither HWCS nor SCCS altered locomotor activity in the open field test, excluding the possibility that the effect of SCCS is due to activation of locomotion. Pretreatment with prazosin (an adrenoreceptor antagonist) or sulpiride (a dopamine D2 receptor antagonist) reduced the effect of SCCS on the immobility time. In contrast, pretreatment with p-chlorophenylalanine (p-CPA, a serotonin synthesis inhibitor) did not alter the anti-immobility effect of SCCS. The last finding is consistent with an additional observation that SCCS had no effect on head twitch response induced by 5-hydroxy-L-tryptophan in mice. Taken altogether, these results suggest that SCCS may elicit an antidepressant-like effect by affecting the adrenergic and doparninergic systems, but not by affecting the serotonergic system.