Cloning and expression analysis of heat-shock transcription factor gene CaHsfA2 from pepper (Capsicum annuum L.)
Cloning and expression analysis of heat-shock transcription factor gene CaHsfA2 from pepper (Capsicum annuum L.)
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DOI：
10.4238/2014.march.17.14
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发表时间：
2014-01-01
影响因子：
0.4
通讯作者：
Gong, Z. -H.
中科院分区：
文献类型：
--
作者：
Guo, M.;Yin, Y. -X.;Gong, Z. -H.
热休克转录因子（Hsf）基因CaHsfA2 (GenBank登录号：利用电子克隆技术和cDNA末端快速扩增技术，从辣椒耐热系R9中克隆出JX402923基因。该基因长1436 bp，开放阅读框长1089 bp，编码362个氨基酸。cDNA的321位和322位之间有一个831 bp的内含子。推导出的CaHsfA2氨基酸序列包含Hsf的保守结构域，包括DNA结合结构域、具有七肽疏水重复序列的邻近结构域（A/B）、激活子基序、核定位信号和核输出信号，其假设注释的E值最高。CaHsfA2与番茄（Lycopersicon peruvianum）和麻瓜（Mimulus guttatus）的HsfA2亲缘关系最近，与植物分类一致。在40℃热激处理2 h后，在耐热品种R9和热敏品种B6的不同组织中观察到CaHsfA2的表达；然而，CaHsfA2基因的表达水平有显著差异，表达在B6叶>茎>花>根，表达在R9花>叶>茎接近根。
The heat-shock transcription factor (Hsf) gene CaHsfA2 (GenBank accession No. JX402923) was cloned from the Capsicum annuum thermotolerant line R9 by combining the techniques electron cloning and rapid amplification of cDNA ends. The gene, which is 1436 bp in length, had an open reading frame of 1089 bp that encoded 362 amino acids. There was an 831-bp intron between positions 321 and 322 of the cDNA. The deduced amino acid sequence of CaHsfA2 contained the conserved domains of Hsf, including DNA binding domain, adjacent domain with heptad hydrophobic repeats (A/B), activator motifs, nuclear localization signal, and nuclear export signal, and it had the highest E value of hypothesized annotation of HsfA2. CaHsfA2 had the nearest phylogenetic relationship with HsfA2 from Lycopersicon peruvianum and Mimulus guttatus, which was consistent with its botanical classification. After heat-shock treatment at 40 C for 2 h, the expression of CaHsfA2 was observed in different tissues of thermotolerant cultivar R9 and thermosensitive line B6; however, the expression levels of the CaHsfA2 gene were significantly different as follows: expression in B6 leaf > stem > flower > root, and expression in R9 flower > leaf > stem approximate to root.