CHARACTERIZATION AND MUTAGENESIS OF THE GENE ENCODING THE A49 SUBUNIT OF RNA POLYMERASE-A IN SACCHAROMYCES-CEREVISIAE
CHARACTERIZATION AND MUTAGENESIS OF THE GENE ENCODING THE A49 SUBUNIT OF RNA POLYMERASE-A IN SACCHAROMYCES-CEREVISIAE
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DOI：
10.1073/pnas.89.19.9302
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发表时间：
1992-10-01
影响因子：
11.1
通讯作者：
SENTENAC, A
中科院分区：
文献类型：
--
作者：
LILJELUND, P;MARIOTTE, S;SENTENAC, A
在酿酒酵母中，RNA聚合酶A的49-kDa亚基的编码基因已经通过在S.基于这两个物种中RNA聚合酶A亚基之间存在的多态性，对酿酒酵母A49亚基和道格拉斯酵母亚基进行了比较。该基因的序列揭示了一种具有异常高赖氨酸含量的碱性蛋白质，这可能是该亚基对DNA表现出亲和力的原因。与任何聚合酶亚基、酶或转录因子没有明显的同源性，单拷贝RPA 49基因的完全缺失导致有活力但生长缓慢的菌落。将HIS 3基因插入RPA 49编码区的一半，导致合成截短的A49亚基，其被掺入聚合酶中。截短的和野生型亚基在杂合二倍体中平等地竞争组装，尽管野生型在表型上是显性的。
The gene encoding the 49-kDa subunit of RNA polymerase A in Saccharomyces cerevisiae has been identified by formation of a hybrid enzyme between the S. cerevisiae A49 subunit and Saccharomyces douglasii subunits based on a polymorphism existing between the subunits of RNA polymerase A in these two species. The sequence of the gene reveals a basic protein with an unusually high lysine content, which may account for the affinity for DNA shown by the subunit. No appreciable homology with any polymerase subunits, enzymes, or transcription factors is found. Complete deletion of the single-copy RPA49 gene leads to viable but slowly growing colonies. Insertion of the HIS3 gene halfway into the RPA49 coding region results in synthesis of a truncated A49 subunit that is incorporated into the polymerase. The truncated and wild-type subunits compete equally for assembly in the heterozygous diploid, although the wild type is phenotypically dominant.