Effects of fenoxycarb on the secretory activity of the prothoracic glands in the fifth instar of the silkworm, Bombyx mori.
Effects of fenoxycarb on the secretory activity of the prothoracic glands in the fifth instar of the silkworm, Bombyx mori.
复制标题
苯氧威对第五龄家蚕前胸腺分泌活性的影响。
DOI：
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发表时间：
1996
影响因子：
2.7
通讯作者：
H. Fugo
中科院分区：
文献类型：
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作者：
S. G. Dedos;H. Fugo
研究了甲氧威对家蚕前胸腺(PG)分泌活性的影响。苯氧威在体外对PGs的分泌活性在整个5龄都有抑制作用。在含有1微克杀菌威的PG中孵育后，其分泌活性大约被抑制了50%。甲氧威的这种抑制作用具有剂量依赖性。重组促胸腺激素(RPTTH)刺激PG的激活率高达15.9。同时给予甲氧威和1 ng rPTTH可显著降低rPTTH对PG的刺激作用，激活比从未超过3.65。在5龄初期，甲氧威强烈抑制虫体的分泌活动，并引起发育停滞。在开始化蛹后，其对PG分泌活性的作用是抑制的。然而，尽管在头部结扎的动物中可以这样做，但在幼虫承诺后，苯氧碳酸酯治疗并不能诱导完整动物的发育停滞。反之，在游荡行为后使用杀灭威能显著增加完整幼虫和头部结扎幼虫的PGs的分泌活性。
Effects of fenoxycarb on the secretory activity of the prothoracic glands (PGs) of the silkworm, Bombyx mori, were investigated. Fenoxycarb inhibited the secretory activity of PGs in vitro throughout the fifth instar. Incubation of PGs in the presence of 1 microgram of fenoxycarb resulted in a approximately 50% inhibition of their secretory activity. This inhibition of fenoxycarb was dose dependent. Stimulation of the PGs by recombinant prothoracicotropic hormone (rPTTH) reached a high activation ratio of 15.9. Simultaneous presence of fenoxycarb and 1 ng rPTTH significantly decreased the stimulatory effect of rPTTH on the PGs, and the activation ratio never exceeded 3.65. Early in the fifth instar, fenoxycarb strongly inhibited the secretory activity of the PGs and induced a developmental arrest as well. After the onset of pupal commitment, its action toward the PG secretory activity was inhibitory. However, fenoxycarb treatment was not able to induce a developmental arrest in intact animals after the pupal commitment although it could do so in head-ligated animals. On the contrary, application of fenoxycarb after the wandering behavior resulted in a significantly increased secretory activity by the PGs of both intact and head-ligated larvae.