A VARIANT BETA-TUBULIN ISOFORM OF DROSOPHILA-MELANOGASTER (BETA-3) IS EXPRESSED PRIMARILY IN TISSUES OF MESODERMAL ORIGIN IN EMBRYOS AND PUPAE, AND IS UTILIZED IN POPULATIONS OF TRANSIENT MICROTUBULES
A VARIANT BETA-TUBULIN ISOFORM OF DROSOPHILA-MELANOGASTER (BETA-3) IS EXPRESSED PRIMARILY IN TISSUES OF MESODERMAL ORIGIN IN EMBRYOS AND PUPAE, AND IS UTILIZED IN POPULATIONS OF TRANSIENT MICROTUBULES
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DOI：
10.1016/s0012-1606(89)80014-4
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发表时间：
1989-02-01
影响因子：
2.7
通讯作者：
RAFF, EC
中科院分区：
文献类型：
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作者：
KIMBLE, M;INCARDONA, JP;RAFF, EC
果蝇β-3-微管蛋白基因编码一种不同的微管蛋白，在发育过程中有两个不同的时间表达：(1)受精后8-16小时的中期胚胎发育；(2)幼虫发育的4天。我们通过使用3‘’消息特异性RNA探针定位石蜡切片中的β3-微管蛋白和使用针对Drosphila的多克隆抗体定位β3-微管蛋白来确定β3-微管蛋白的空间表达模式。在胚胎发育过程中，β3仅限于所有发育中的肌肉，并在所有发育的肌肉中表达。在蛹发育期间，β3在发育中的成虫肌肉中也有高水平的表达。此外，在蛹发育的早期，β3在成虫的视盘中表达，而在后期，β3在翅叶、视叶、卵巢和睾丸的表皮细胞中表达。在这些组织中，β3微管蛋白的表达在蛹发育结束时停止，但卵巢和睾丸中的表达持续到成虫。我们的结果表明，在发育中的肌肉和翅膀中，β3-微管蛋白被用于特殊但短暂的细胞骨架微管群体中，这些微管参与建立组织的最终形式。
The .beta.3-tubulin gene of Drosophila melanogaster codes for a variant tubulin which is expressed at two distinct times during development: (1) during midembryogenesis from 8-16 hr postfertilization, and (2) during the 4 days of pupal development. We have determined the spatial pattern of .beta.3-tubulin expression by localizing the .beta.3 mRNA in paraffin sections using a 3'' message-specific RNA probe and by localizing the .beta.3 protein using a polyclonal antibody specific for Drosphila .beta.3-tubulin. During embryogenesis .beta.3 is restricted to and is expressed in all of the developing muscles. During pupal development .beta.3 is also expressed at high levels in developing adult muscles. In addition, early in pupal development .beta.3 is expressed in the imaginal discs, while at later times .beta.3 is expressed in the epidermal cells of the wing blade, the optic lobe, the ovaries, and the testes. The expression of .beta.3 tubulin ceases by the end of pupal developmentt in all of these tissues except the ovaries and testes where expression persists into the adult. In both developing muscles and wings our results indicate that .beta.3-tubulin is utilized in populations of specialized but transient cytoskeletal microtubules which are involved in establishing the final form of the tissue.