B7-H3 enhances tumor immunity in vivo by costimulating rapid clonal expansion of antigen-specific CD8+ cytolytic T cells
B7-H3 enhances tumor immunity in vivo by costimulating rapid clonal expansion of antigen-specific CD8+ cytolytic T cells
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DOI：
10.4049/jimmunol.173.9.5445
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发表时间：
2004-11-01
影响因子：
4.4
通讯作者：
Chen, LP
中科院分区：
文献类型：
--
作者：
Luo, LQ;Chapoval, AI;Chen, LP
B7-H3是体外具有T细胞共刺激功能的B7家族分子。B7-H3在体内对肿瘤免疫的刺激作用尚不清楚。我们在此报道，通过将B7-H3基因导入小鼠P815肿瘤细胞系，可增强其免疫原性，导致肿瘤消退，并在同基因小鼠中扩增肿瘤特异性CD8(+)CTL反应。表达B7-H3基因的肿瘤细胞可在体内诱导P1a肿瘤抗原特异性CD8(+)CTL在体内的快速克隆性扩增，并在体外获得直接刺激T细胞生长、分裂和发展杀伤活性的能力。因此，我们的结果确立了B7-H3在体内共刺激T细胞免疫反应中的作用。
B7-H3 is a B7 family molecule with T cell costimulatory function in vitro. The in vivo role of B7-H3 in the stimulation of tumor immunity is unclear. We report here that expression of B7-H3 by transfection of the mouse P815 tumor line enhances its immunogenicity, leading to the regression of tumors and amplification of a tumor-specific CD8(+) CTL response in syngeneic mice. Tumor cells engineered to express B7-H3 elicit a rapid clonal expansion of P1A tumor Ag-specific CD8(+) CTL in lymphoid organs in vivo and acquire the ability to directly stimulate T cell growth, division, and development of cytolytic activity in vitro. Our results thus establish a role for B7-H3 in the costimulation of T cell immune responses in vivo.