Diversity and distribution of 16S rRNA and phenol monooxygenase genes in the rhizosphere and endophytic bacteria isolated from PAH-contaminated sites.
Diversity and distribution of 16S rRNA and phenol monooxygenase genes in the rhizosphere and endophytic bacteria isolated from PAH-contaminated sites.
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PAH污染地根际和内生细菌16S rRNA和苯酚单加氧酶基因的多样性和分布
DOI：
10.1038/srep12173
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发表时间：
2015-07-17
影响因子：
4.6
通讯作者：
Gao Y
中科院分区：
文献类型：
--
作者：
Peng A;Liu J;Ling W;Chen Z;Gao Y
这是首次对多环芳烃（PAHs）不同污染水平场地的植物内生菌和根际细菌中16S rRNA和苯酚单加氧酶（PHE）基因的多样性及分布进行的研究。在看麦娘（Alopecurus aequalis Sobol）和酢浆草（Oxalis corniculata L.）的根际土壤中，分别检测到浓度为34.22 - 55.29和45.79 - 97.81 mg·kg⁻¹的10种多环芳烃。基于PCR - DGGE数据显示，根际土壤或植物中16S rRNA和PHE基因的多样性随多环芳烃污染水平的变化而改变。一般来说，在轻度或中度污染区域发现香农 - 维纳指数较高。从根际土壤中获得了总共82条不同的细菌16S rRNA基因序列，它们属于5个门，即放线菌门（Acfinobacteria，应为Actinobacteria）、变形菌门、绿弯菌门、蓝藻门和拟杆菌门。对于已鉴定的57条PHE基因序列，其中18条来自根际细菌，39条来自内生细菌。根际细菌和内生细菌中16S rRNA和PHE基因的拷贝数分别在3.83×10³ - 2.28×10⁶和4.17×10² - 1.99×10⁵之间变化。根际细菌中PHE基因的拷贝数显著高于内生细菌。研究结果增进了我们对多环芳烃污染场地生长的植物的根际和内生细菌多样性的了解。
This is the first investigation of the diversity and distribution of 16S rRNA and phenol monooxygenase (PHE) genes in endophytic and rhizosphere bacteria of plants at sites contaminated with different levels of PAHs. Ten PAHs at concentrations from 34.22 to 55.29 and 45.79 to 97.81 mg·kg−1 were measured in rhizosphere soils of Alopecurus aequalis Sobol and Oxalis corniculata L., respectively. The diversity of 16S rRNA and PHE genes in rhizosphere soils or plants changed with varying PAH pollution levels, as shown based on PCR-DGGE data. Generally, higher Shannon-Weiner indexes were found in mild or moderate contaminated areas. A total of 82 different bacterial 16S rRNA gene sequences belonging to five phyla; namely, Acfinobacteria, Proteobacteria, Chloroflexi, Cyanophyta, and Bacteroidetes, were obtained from rhizosphere soils. For the 57 identified PHE gene sequences, 18 were excised from rhizosphere bacteria and 39 from endophytic bacteria. The copy numbers of 16S rRNA and PHE genes in rhizosphere and endophytic bacteria varied from 3.83 × 103 to 2.28 × 106 and 4.17 × 102 to 1.99 × 105, respectively. The copy numbers of PHE genes in rhizosphere bacteria were significantly higher than in endophytic bacteria. Results increase our understanding of the diversity of rhizosphere and endophytic bacteria from plants grown in PAH-contaminated sites.