Changes of N/L ratio and cortisol levels associated with experimental training in untrained rhesus macaques
Changes of N/L ratio and cortisol levels associated with experimental training in untrained rhesus macaques
复制标题
DOI：
10.1111/jmp.12023
复制
发表时间：
2013-02-01
影响因子：
0.7
通讯作者：
Kim, Sang-Joon
中科院分区：
文献类型：
--
作者：
Lee, Jae-Il;Shin, Jun-Seop;Kim, Sang-Joon
实验前的动物训练是一个非常重要的过程，因为它可能影响血液学和激素变量的结果以及受这些因素影响的功能。由于这种训练不可避免地会给动物带来压力，我们想知道未经训练的猴子需要多长时间才能从实验训练带来的压力中恢复过来。方法对新获得的恒河猴进行实验性猴椅训练前后的血液学和应激激素（如皮质醇）变化进行每周监测。结果中性粒细胞/淋巴细胞（N/L）比值在训练初期显著升高，3周后逐渐降低。初始阶段升高的血清皮质醇水平在3周的椅子训练后也显著下降。结论猴子椅训练至少需要3周的时间才能从训练压力中恢复。这些结果表明，许多使用非人类灵长类动物的研究人员在开始研究之前应该提供足够的时间（大约3周）来适应实验环境。
Background Animal training prior to beginning an experiment is an essential procedure and a very important course because it may affect the results of hematologic and hormonal variables and the functions influenced by those factors. Because this training inevitably causes animal stress, we wondered how much time is needed for untrained monkeys to recover from stresses associated with experimental training. Methods We measured the hematological and stress hormonal (e.g., cortisol) changes on weekly basis before and after experimental monkey chair training in newly acquired rhesus monkeys. Results The neutrophil-to-lymphocyte (N/L) ratio significantly increased during the initial phase of the training and then gradually decreased after 3 weeks. Elevated serum cortisol levels in the initial phase also significantly decreased after 3 weeks of chair training. Conclusions We conclude that at least a 3-week period is needed in monkey chair training for recovery from training stress. These results suggest that many researchers using nonhuman primates should provide enough time (>3 weeks) for adaptation to the experimental environment prior to beginning a study.