Mutations of acetylcholinesterase1 contribute to prothiofos-resistance in Plutella xylostella (L.)
Mutations of acetylcholinesterase1 contribute to prothiofos-resistance in Plutella xylostella (L.)
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DOI：
10.1016/j.bbrc.2006.12.088
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发表时间：
2007-02-16
影响因子：
3.1
通讯作者：
Koh, Young Ho
中科院分区：
文献类型：
--
作者：
Lee, Dae-Weon;Choi, Jae Young;Koh, Young Ho
不敏感乙酰胆碱酯酶（AChE）参与了有机磷和氨基甲酸酯类杀虫剂的抗性。从小菜蛾（Plutella xylostella）成虫头中克隆了一段新的编码ace1基因的全长乙酰胆碱酯酶cDNA。ace1基因编码679个氨基酸，具有催化三联体、胆碱结合位点和酰基袋等保守基序。Northern blot分析显示，在所有检测的身体部位，ace1基因的表达量远高于ace2。表达的乙酰硫代胆碱对乙酰硫代胆碱具有底物特异性，对BW284C51和eserine具有抑制剂特异性。在抗硫磷菌株中鉴定出3个AChE1突变（D229G、A298S和G324A），通过三维建模推测其中2个突变（A298S和G324A）可能与抗硫磷有关。SO细胞中ache的体外功能表达显示，对氧磷对只有耐药AChE1的抑制作用较弱，提示耐药AChE1与抗硫磷有关。(c) 2006爱思唯尔公司版权所有。
Insensitive acetylcholinesterase (AChE) is involved in the resistance of organophosphorous and carbamate insecticides. We cloned a novel full-length AChE cDNA encoding ace1 gene from adult heads of the diamondback moth (DBM, Plutella xylostella). The ace1 gene encoding 679 amino acids has conserved motifs including catalytic triad, choline-binding site and acyl pocket. Northern blot analysis revealed that the ace1 gene was expressed much higher than the ace2 in all examined body parts. The biochemical properties of expressed AChEs showed substrate specificity for acetylthiocholine iodide and inhibitor specificity for BW284C51 and eserine. Three mutations of AChE1 (D229G, A298S, and G324A) were identified in the prothiofos-resistant strain, two of which (A298S and G324A) were expected to be involved in the prothiofos-resistance through three-dimensional modeling. In vitro functional expression of AChEs in SO cells revealed that only resistant AChE1 is less inhibited with paraoxon, suggesting that resistant AChE1 is responsible for prothiofos-resistance. (c) 2006 Elsevier Inc. All rights reserved.