Asymmetric distribution of carbonic anhydrase in the alveolar-capillary barrier.
Asymmetric distribution of carbonic anhydrase in the alveolar-capillary barrier.
复制标题
碳酸酐酶在肺泡毛细血管屏障中的不对称分布。
DOI：
10.1152/jappl.1981.51.1.190
复制
发表时间：
1981
期刊：
影响因子：
--
通讯作者：
Silverman,P
中科院分区：
文献类型：
--
作者：
Effros,RM;Mason,G;Silverman,P
用0.5ml NaH_（14）CO_3和~ 3 H_2 O的缓冲液注入灌注兔肺的远端气道，研究肺碳酸酐酶（CA）活性。肺静脉流出中14 C的早期恢复平均为对照情况下3 H的0.28 +/- 0.02（SE）。在灌注液中加入CA抑制剂（20 mg/l乙酰唑胺）使14 C回收率相对于3 H降低了三分之二，而这种抑制剂在气道注射液中没有效果。在灌注液中加入100 mg/l Ca并没有增加14 C的回收率，但在支气管液中加入酶使14 C的回收率相对于3 H增加了5倍以上。它的结论是，CA是与肺泡毛细血管屏障的内皮侧，但不存在的气道表面。
Pulmonary carbonic anhydrase (CA) activity was studied by injecting 0.5 ml of a buffered solution of NaH14CO3 and 3H2O into the distal airways of perfused rabbit lungs. The early recovery of 14C in the pulmonary venous outflow averaged 0.28 +/- 0.02 (SE) that of 3H under control circumstances. Incorporation of a CA inhibitor (20 mg/l acetazolamide) in the perfusate reduced 14C recovery relative to 3H by two-thirds, whereas this inhibitor had no effect when placed in the airway injection fluid. Addition of 100 mg/l Ca to the perfusate did not increase 14C recovery, but addition of the enzyme to the bronchial fluid increased 14C recovery relative to 3H by a factor of more than 5. It is concluded that CA is associated with the endothelial side of the alveolar-capillary barrier but is absent on the airway surfaces.