Selected Th1 and Th2 cytokine mRNA expression by CD4(+) T cells isolated from inflamed human gingival tissues.
Selected Th1 and Th2 cytokine mRNA expression by CD4(+) T cells isolated from inflamed human gingival tissues.
复制标题
从发炎的人牙龈组织中分离的 CD4(+) T 细胞表达选定的 Th1 和 Th2 细胞因子 mRNA。
DOI：
10.1111/j.1365-2249.1996.tb08297.x
复制
发表时间：
1996
影响因子：
4.6
通讯作者：
Kiyono,H
中科院分区：
文献类型：
--
作者：
Fujihashi,K;Yamamoto,M;Hiroi,T;Bamberg,TV;McGhee,JR;Kiyono,H
浆细胞数量的增加与成人牙周炎患者的局限性和慢性炎症有关。然而，目前仅有有限的信息表明，在受影响的牙龈组织中，由CD4+T细胞产生的细胞因子如何参与这些增加的B细胞反应。从炎症组织中分离出单个核细胞，经流式细胞仪检测，CD4+T细胞占∼+T细胞的20-30%。用干扰素-γ和IL-2、IL-4、IL-5、IL-6、IL-10和IL-13(内对照)的特异性5‘和3’引物行逆转录聚合酶链式反应(RT-γ)检测Th1和Th2细胞因子的表达。根据所选择的Th1和Th2细胞因子的表达，发现了两种不同的细胞因子谱，一种是干扰素-γ、IL-6、IL-10和IL-13的表达，另一种是干扰素-γ、IL-6和IL-13的表达。从刀豆蛋白A刺激的外周血单个核细胞(PBMC)中提取RNA，进行RT-PCR检测。检测所有Th1和Th2细胞因子的mRNA表达。这些发现表明，尽管人类CD_4+T细胞能够产生一系列Th1型和Th2型细胞因子，但与牙周炎相关的CD_4+T细胞仅限于产生干扰素-γ、IL-6、IL-13，在某些情况下，还可产生IL-10。根据是否产生IL-10，以及干扰素-γ、IL-6和IL-13，牙周组织中的CD_4+T细胞可分为两组。
Elevated numbers of plasma cells are associated with localized and chronically inflamed gingiva of patients with adult periodontitis. However, only limited information is currently available as to how cytokines produced by CD4+T cells are involved in these increased B cell responses in affected gingival tissues. When gingival mononuclear cells (GMC) were isolated from inflamed tissues and examined by flow cytometry, ∼20–30% of lymphocytes were CD4+T cells. For the analysis of Th1 and Th2 cytokine expression by these CD4+T cells, RNA was extracted and reverse transcriptase‐polymerase chain reaction (RT‐PCR) was performed by using specific 5' and 3' primers for interferon‐gamma (IFN‐γ) and IL‐2 (Th1), IL‐4, IL‐5, IL‐6, IL‐10 and IL‐13 (Th2) and β‐actin (internal control). Two distinct cytokine profiles were noted based on the expression of selected Th1 and Th2 cytokines, where one pattern was represented by expression of mRNA for IFN‐γ, IL‐6, IL‐10 and IL‐13, while the second consisted of mRNA for IFN‐γ, IL‐6 and IL‐13. In most samples, mRNA for IL‐2, IL‐4 and IL‐5 were not detected by cytokine‐specific RT‐PCR. When RNA was isolated from CD4+T cells of concanavalin A‐stimulated peripheral blood mononuclear celts (PBMC) of the same patients and examined by RT‐PCR. mRNA for all Th1 and Th2 cytokines were detected. These findings suggest that although human CD4+T cells are capable of producing an array of Th1‐ and Th2‐type cytokines, the CD4+T cells associated with periodontitis are limited to production of IFN‐γ, IL‐6, IL‐13 and in some instances IL‐10. CD4+T cells from diseased periodontal tissues are divisible into two groups based upon whether or not IL‐10 is produced, together with IFN‐γ, IL‐6 and IL‐13.