LOCATION OF CHITIN IN THE CYST WALL OF ENTAMOEBA-INVADENS WITH COLLOIDAL GOLD TRACERS
LOCATION OF CHITIN IN THE CYST WALL OF ENTAMOEBA-INVADENS WITH COLLOIDAL GOLD TRACERS
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DOI：
10.2307/3281183
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发表时间：
1982-01-01
影响因子：
1.3
通讯作者：
CARABEZTREJO, A
中科院分区：
文献类型：
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作者：
ARROYOBEGOVICH, A;CARABEZTREJO, A
几丁质与胶体金结合的麦胚凝集素定位于侵袭性肠杆菌的囊壁中。当包囊培养物用金示踪剂处理时，包囊与滋养体染色不同；包囊呈酒红色，而滋养体一般保持不染。细胞的EM观察表明，细胞悬浮液暴露时，示踪剂颗粒特异性地结合到囊壁表面，而细胞薄片暴露于细胞时，示踪剂颗粒特异地结合到囊壁横截面上，表明甲壳素纤维分布在囊壁表面和整个囊壁基质中。
Chitin was located in the cyst wall of E. invadens with colloidal gold-linked wheat germ agglutinin. Cysts stained differentially from trophozoites when encysting cultures were treated with the gold tracer; cysts acquired a wine-red coloration while, in general, trophozoites remained unstained. Observation of cells by EM revealed that the tracer particles were bound specifically to the walls of the surface of the cyst when cells were exposed in suspension and to the cyst wall cross section, when cells were exposed to the tracer in thin section, indicating that chitin fibers were distributed on the surface as well as throughout the matrix of the cyst wall.