Immunogenicity and anti-fecundity effect of nanoparticle coated glutathione S-transferase (SjGST) DNA vaccine against murine Schistosoma japonicum infection.
Immunogenicity and anti-fecundity effect of nanoparticle coated glutathione S-transferase (SjGST) DNA vaccine against murine Schistosoma japonicum infection.
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纳米颗粒包被的谷胱甘肽 S-转移酶 (SjGST) DNA 疫苗对小鼠日本血吸虫感染的免疫原性和抗生殖作用。
DOI：
10.1016/j.parint.2015.01.005
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发表时间：
2015
期刊：
影响因子：
--
通讯作者：
Hirayama K.
中科院分区：
文献类型：
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作者：
Mbanefo EC;Kumagai T;Kodama Y;Kurosaki T;Furushima-Shimogawara R;Cherif MS;Mizukami S;Kikuchi M;Huy NT;Ohta N;Sasaki H;Hirayama K.
目前仍然迫切需要一种针对血吸虫病的疫苗，特别是在日本血吸虫流行地区，在这些地区，即使是阻断人畜共患传播的疫苗也可能作为一种有效的干预工具。我们开发了一种新型的纳米颗粒基因传递系统，该系统已被证明可以有效地靶向免疫细胞进行基因转染，具有互补的佐剂作用，对多种疾病具有很高的保护作用。在这里，我们将这种纳米颗粒系统与s结合使用。研究日本谷胱甘肽s -转移酶（SjGST） DNA疫苗的免疫原性和抗鼠血吸虫病纳米颗粒包被疫苗制剂的抗生育作用。纳米颗粒包被的DNA疫苗制剂诱导了预期的免疫反应。与纳米颗粒包被的空载体相比，它产生了明显增加的抗原特异性体液反应，t -辅助性1极化细胞因子环境，产生IFN-γ的CD4+ t细胞比例更高，同时产生IL-4的CD4+ t细胞比例降低。虽然对蠕虫负荷没有影响，但我们记录到组织卵负荷明显减少。雌成虫组织卵负担降低71.3%，繁殖力降低55%。我们的数据显示，使用纳米颗粒基因递送系统的sjgst DNA疫苗对雌性成年血吸虫产生了与传统亚单位疫苗加佐剂相同的抗生殖力作用，证明该DNA疫苗制剂具有抗病理和阻断传播应用的前景。
There is still urgent need for a vaccine against schistosomiasis, especially inSchistosoma japonicumendemic areas where even a vaccine that will interrupt zoonotic transmission will be potentially effective as an intervention tool. We had developed a novel nanoparticle gene delivery system, which has proven efficacious in gene transfection to target immune cells with complementary adjuvant effect and high protective efficacy in several diseases. Here, we applied this nanoparticle system in combination withS.japonicumglutathione S-transferase (SjGST) DNA vaccine to show the immunogenicity and anti-fecundity effect of the nanoparticle coated vaccine formulation against murine schistosomiasis. The nanoparticle-coated DNA vaccine formulation induced desired immune responses. In comparison with the nanoparticle coated empty vector, it produced significantly increased antigen-specific humoral response, T-helper 1 polarized cytokine environment, higher proportion of IFN-γ producing CD4+T-cells and the concomitant decrease in IL-4 producing CD4+T-cells. Although there was no effect on worm burden, we recorded a marked reduction in tissue egg burden. There was up to 71.3% decrease in tissue egg burden and 55% reduction in the fecundity of female adult worms. Our data showed thatSjGST DNA vaccine, delivered using the nanoparticle gene delivery system, produced anti-fecundity effect on female adult schistosomes as previously described by using conventional subunit vaccine with adjuvant, proving this DNA vaccine formulation as a promising candidate for anti-pathology and transmission blocking application.