Distribution of the pressure-regulated operons in deep-sea bacteria
Distribution of the pressure-regulated operons in deep-sea bacteria
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DOI：
10.1016/s0378-1097(97)00560-0
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发表时间：
1998-02-15
影响因子：
2.1
通讯作者：
Horikoshi, K
中科院分区：
文献类型：
--
作者：
Li, LN;Kato, C;Horikoshi, K
先前在两种深海嗜盐细菌中发现的两个不同的压力调节操纵子对应的DNA区域分别从各种深海微生物中扩增并测序。使用两组引物，从检查的嗜盐性细菌中扩增特别成功。16S rRNA序列分析表明，这些细菌在系统发育上具有亲缘关系，属于希瓦氏菌属的一个亚分支，仅包含深海希瓦氏菌嗜水细菌。我们将这个分支定义为包含至少两个不同物种的“希瓦氏菌嗜盐分支”。结果表明，压力调控操纵子的DNA序列可作为嗜盐希瓦氏菌菌株鉴定的标记序列。(C) 1998年欧洲微生物学会联合会。Elsevier Science B.V.出版
DNA regions corresponding to portions of two different pressure-regulated operons previously identified in two deep-sea barophilic bacteria were separately PCR amplified from a variety of deep-sea microorganisms and sequenced. With the two sets of primers employed, amplification was particularly successful from the more barophilic bacteria examined. 16S rRNA sequence analysis revealed that these bacteria are all phylogenetically related and belong in a sub-branch of the genus Shewanella containing only the deep-sea Shewanella barophilic bacteria. We define this sub-branch as the 'Shewanella barophile branch' containing at least two different species. Our results suggest that the DNA sequences of the pressure-regulated operons can be regarded as marker sequences to identify the Shewanella barophilic strains. (C) 1998 Federation of European Microbiological Societies. Published by Elsevier Science B.V.