Sequence of the human GATA-1 promoter.
Sequence of the human GATA-1 promoter.
复制标题
人类 GATA-1 启动子的序列。
DOI：
10.1093/nar/20.7.1812
复制
发表时间：
1992
影响因子：
14.9
通讯作者：
Orkin,SH
中科院分区：
文献类型：
--
作者：
Zon,LI;Orkin,SH
The hematopoietic-specific transcription factor GATA-1 participates in control of erythroid-expressed genes (1) and is required for erythroid development in vivo (2). Recently Nicolis et al.(3) reported human GATA-1 gene promoter sequences. Homology to mouse GATA-1 sequences (2) was noted only in the proximal promoter and in an upstream region containing a double GATA-site implicated in autoregulation (4). Their sequence of the human GATA-1 promoter differs from that we have obtained with respect to the omission of 121 bp, which display substantial similarity with the mouse (Figure 1, bracketed region). Confirmation that this region is indeed part of the promoter was achieved by PCR (Figure 2) using two independent human GATA-l genomic clones (lanes 1, 2, 8, 9) and total human DNA samples (lanes 3-7). The revisedhuman promoter sequence brings the two mammalian sequences into closer alignment (Figure 2), and predicts strong functional similarities between human and mouse GATA-1 regulatory regions.
