SPECIFIC LABELING OF INTRACELLULAR TOXOPLASMA-GONDII WITH URACIL
SPECIFIC LABELING OF INTRACELLULAR TOXOPLASMA-GONDII WITH URACIL
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DOI：
10.1111/j.1550-7408.1977.tb04774.x
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发表时间：
1977-01-01
期刊：
影响因子：
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通讯作者：
PFEFFERKORN, LC
中科院分区：
文献类型：
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作者：
PFEFFERKORN, ER;PFEFFERKORN, LC
放射性尿嘧啶未显著掺入人成纤维细胞的核酸中。用T.弓形虫导致尿嘧啶掺入率的指数增加，这抑制了寄生虫的指数生长。感染后一天，尿嘧啶掺入率增加100倍。放射自显影显示，所有的[3H]尿嘧啶被纳入细胞内的寄生虫。这种利用尿嘧啶能力的差异可能是由于尿苷磷酸化酶的比活性在部分纯化的寄生虫中比在宿主细胞中高100倍。
Radioactive uracil was not significantly incorporated into the nucleic acids of human fibroblast cells. Infection of these cells with T. gondii resulted in an exponential increase in the rate of uracil incorporation that paralleled the exponential growth of the parasite. One day after infection the rate of uracil incorporation was increased 100-fold. Autoradiography showed that all of the [3H] uracil was incorporated into the intracellular parasites. This difference in ability to use uracil may be due to the specific activity of uridine phosphorylase being 100-fold greater in partially purified parasites than in the host cell.