Preliminary studies on induction of apoptosis by genistein on HepG2 cell line
Preliminary studies on induction of apoptosis by genistein on HepG2 cell line
复制标题
DOI：
10.1016/j.tiv.2007.01.023
复制
发表时间：
2007-08-01
影响因子：
3.2
通讯作者：
Sakthisekaran, D.
中科院分区：
文献类型：
--
作者：
Chodon, Dechen;Ramamurty, Nalini;Sakthisekaran, D.
大豆制品的消费与降低癌症发病率有关。染料木黄酮是主要的大豆异黄酮之一，在体外已显示出抑制许多肿瘤细胞系的生长。本研究通过观察细胞凋亡小体的形成和DNA梯状条带的形成，探讨染料木黄酮对人肝癌HepG2细胞生长和凋亡的影响。此外，流式细胞仪分析与碘化丙啶染色已进行检测凋亡细胞。我们发现抑制细胞生长和凋亡的细胞核，DNA断裂和增加的凋亡细胞与染料木素治疗后，表明凋亡细胞死亡。从这些结果中，我们观察到金雀异黄素抑制HepG2细胞的生长和诱导凋亡，然而，需要进一步的确定性研究。这些结果可能支持染料木黄酮对肝癌的潜在有效的化学预防和/或化学治疗。(c)2007年由Elsevier Ltd.出版
Consumption of soy products has been linked to lower the incidence of number of cancers. Genistein, one of the principal soy isoflavones, has been shown to inhibit the growth of a number of tumor cell lines in vitro. In this study, we investigate the effects of genistein on cell growth and apoptosis in human hepatocellular carcinoma HepG2 cell by looking for the formation of nuclear apoptotic bodies and DNA ladder formation. Additionally, flow cytometry analysis with propidium iodide staining has been conducted to detect the apoptotic cells. We found inhibition of cell growth and apoptotic nuclei, DNA fragmentation and increased apoptotic cells after treatment with genistein, indicating apoptotic cell deaths. From these results we observed that genistein inhibits the growth of HepG2 cells and induce apoptosis, however, further definitive studies are needed. These results may support the potentially effective chemopreventive and/or chemotherapeutic of genistein against liver cancer. (c) 2007 Published by Elsevier Ltd.