Oak root response to ectomycorrhizal symbiosis establishment: RNA-Seq derived transcript identification and expression profiling.
Oak root response to ectomycorrhizal symbiosis establishment: RNA-Seq derived transcript identification and expression profiling.
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DOI：
10.1371/journal.pone.0098376
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Paulo OS
中科院分区：
文献类型：
--
作者：
Sebastiana M;Vieira B;Lino-Neto T;Monteiro F;Figueiredo A;Sousa L;Pais MS;Tavares R;Paulo OS
外生菌根共生对温带和北方森林树木的生命和健康至关重要，在森林生态系统的养分循环和功能中起着重要作用。具有外生菌根根尖的树木对环境胁迫（如干旱）和生物胁迫（如根病原体）的耐受性更强。关于这些分子过程的详细信息对于理解共生组织发育以优化这种自然现象的益处至关重要。下一代测序工具允许分析非模型外生菌根植物真菌的相互作用，可以有助于找到“共生工具包”，更好地定义每个合作伙伴在互惠互动的作用。通过使用454焦磷酸测序，我们比较了外生菌根栓皮栎根与非共生根。从测序的两个cDNA文库中，获得了超过200万个读段，产生了19552个栓皮栎根独特的转录本。共发现2238转录差异表达时，ECM根与非共生根进行了比较。外生菌根根中上调和下调基因的鉴定导致了对这一重要共生关系的分子机制的深入了解。在栓皮栎根，外生菌根定植导致广泛的细胞壁重塑，激活的分泌途径，类黄酮的生物合成的改变，并参与识别真菌效应子的基因的表达。此外，我们确定了在共生过程中具有假定作用的基因，如与真菌伙伴的营养交换，侧根形成或根毛腐烂。这些发现提供了一个全球性的概述外生菌根宿主根的转录组，并构成了未来的研究控制这一重要的共生的分子事件的基础。
Ectomycorrhizal symbiosis is essential for the life and health of trees in temperate and boreal forests where it plays a major role in nutrient cycling and in functioning of the forest ecosystem. Trees with ectomycorrhizal root tips are more tolerant to environmental stresses, such as drought, and biotic stresses such as root pathogens. Detailed information on these molecular processes is essential for the understanding of symbiotic tissue development in order to optimize the benefits of this natural phenomenon. Next generation sequencing tools allow the analysis of non model ectomycorrhizal plant-fungal interactions that can contribute to find the “symbiosis toolkits” and better define the role of each partner in the mutualistic interaction. By using 454 pyrosequencing we compared ectomycorrhizal cork oak roots with non-symbiotic roots. From the two cDNA libraries sequenced, over 2 million reads were obtained that generated 19552 cork oak root unique transcripts. A total of 2238 transcripts were found to be differentially expressed when ECM roots were compared with non-symbiotic roots. Identification of up- and down-regulated gens in ectomycorrhizal roots lead to a number of insights into the molecular mechanisms governing this important symbiosis. In cork oak roots, ectomycorrhizal colonization resulted in extensive cell wall remodelling, activation of the secretory pathway, alterations in flavonoid biosynthesis, and expression of genes involved in the recognition of fungal effectors. In addition, we identified genes with putative roles in symbiotic processes such as nutrient exchange with the fungal partner, lateral root formation or root hair decay. These findings provide a global overview of the transcriptome of an ectomycorrhizal host root, and constitute a foundation for future studies on the molecular events controlling this important symbiosis.
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影响因子：
4.8
作者：
Barkefors, Irmeli;Fuchs, Peder Fredlund;Kreuger, Johan
通讯作者：
Kreuger, Johan
DOI：
10.1073/pnas.1107912108
发表时间：
2011-08-23
影响因子：
11.1
作者：
Capoen, Ward;Sun, Jongho;Oldroyd, Giles E. D.
通讯作者：
Oldroyd, Giles E. D.
DOI：
10.1073/pnas.0900689106
发表时间：
2009-08-04
影响因子：
11.1
作者：
Barratt, D. H. Paul;Derbyshire, Paul;Smith, Alison M.
通讯作者：
Smith, Alison M.
影响因子：
7.4
作者：
Hancock, Kerry R.;Collette, Vern;Rasmussen, Susanne
通讯作者：
Rasmussen, Susanne
影响因子：
7.4
作者：
Felten, Judith;Kohler, Annegret;Legue, Valerie
通讯作者：
Legue, Valerie