MURINE CD8(+) T-CELLS THAT SPECIFICALLY DELETE AUTOLOGOUS CD4(+) T-CELLS EXPRESSING V-BETA-8 TCR - A ROLE OF THE Q-ALPHA-1 MOLECULE
MURINE CD8(+) T-CELLS THAT SPECIFICALLY DELETE AUTOLOGOUS CD4(+) T-CELLS EXPRESSING V-BETA-8 TCR - A ROLE OF THE Q-ALPHA-1 MOLECULE
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DOI：
10.1016/s1074-7613(95)80079-4
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发表时间：
1995-02-01
期刊：
影响因子：
32.4
通讯作者：
PERNIS, B
中科院分区：
文献类型：
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作者：
JIANG, H;WARE, R;PERNIS, B
不同T细胞亚群上表达的T细胞受体（TCR）所介导的相互作用可能在免疫调节中起作用。为了研究这一观点，我们研究了超抗原诱导的TCR Vβ限制性反应的调节。我们探究了在葡萄球菌肠毒素B（SEB）注射后，CD4⁺Vβ8⁺ T细胞的体内调节是否受CD8⁺ T细胞控制。我们发现，在缺乏CD8⁺ T细胞的小鼠中，未观察到CD4⁺Vβ8⁺ T细胞下调至基线以下。此外，在给予SEB后，出现的CD8⁺ T细胞在体外优先杀伤活化的CD4⁺Vβ8⁺ T细胞亚群，而非CD4⁺Vβ8⁺ T细胞。这种TCR Vβ特异性细胞毒性依赖于β2 -微球蛋白，并且可被抗Qa - 1的抗血清抑制，但不被抗MHC I类分子的抗体抑制。这些数据表明，免疫调节的特异性可能涉及CD8⁺ T细胞对CD4⁺ T细胞上表达的TCR Vβ决定簇和Qa - 1分子的识别。
Interactions mediated by TCRs expressed on different T cell subsets may play a role in immunoregulation. To investigate this idea, we studied the regulation of superantigen-induced TCR Vp-restricted responses. We asked whether the in vivo regulation of CD4(+) V beta 8(+) T cells following SEB injection is controlled by CD8(+) T cells. We found that in mice deficient in CD8(+) T cells, the down-regulation of CD4(+) V beta 8(+) T cells below baseline is not observed. Moreover, following SEB administration, CD8(+) T cells emerge that preferentially kill subpopulations of activated CD4(+) V beta 8(+) but not CD4(+) V beta 8(+) T cells in vitro. This TCR V beta-specific cytotoxicity is dependent on beta 2-microglobulin and is inhibited by antisera specific for Qa-l but not by antibody to MHC class la. These data suggest the idea that the specificity of immune regulation may involve CD8(+) T cell recognition of TCR V beta determinants and Qa-1 molecules expressed on CD4(+) T cells.