Mutation, DNA strand cleavage and nitric oxide formation caused by N-nitrosoproline with sunlight: a possible mechanism of UVA carcinogenicity.
Mutation, DNA strand cleavage and nitric oxide formation caused by N-nitrosoproline with sunlight: a possible mechanism of UVA carcinogenicity.
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N-亚硝基脯氨酸与阳光引起的突变、DNA 链断裂和一氧化氮形成：UVA 致癌的可能机制。
DOI：
10.1093/carcin/23.9.1537
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发表时间：
2002
期刊：
影响因子：
4.7
通讯作者：
Michael H. L. Green
中科院分区：
文献类型：
--
作者：
S. Arimoto;Yoshiko Ando;Y. Horai;K. Okamoto;H. Hayatsu;J. Lowe;Michael H. L. Green
N-亚硝基脯氨酸（NPRO）是由脯氨酸和亚硝酸盐内源形成的。据报告，NPRO无致突变性和致癌性。本研究检测了NPRO加自然日光对鼠伤寒沙门氏菌的直接致突变性。此外，在NPRO加模拟日光处理的小牛胸腺DNA中观察到8-氧代-7，8-二氢-2 '-脱氧鸟苷（8-oxodG）的形成，这是一种致突变性损伤。用NPRO和阳光处理诱导噬菌体M13 mp2 DNA的超螺旋复制形式的单链断裂。彗星试验检测到，在NPRO加UVA处理的人成纤维细胞中也发生了单链DNA断裂。使用清除剂的分析表明，活性氧和NO自由基介导的链断裂。在UVA照射的NPRO溶液中观察到一氧化氮的形成。我们分析了突变诱导和DNA断裂的光动力学光谱，使用单色辐射在300和400 nm之间的一系列波长。突变频率和DNA断裂均在NPRO的最大吸收波长340 nm处最高。NPRO和阳光的共致突变和共毒性作用值得关注，因为这可能是增加UVA照射致癌风险的机制。
N-Nitrosoproline (NPRO) is endogenously formed from proline and nitrite. NPRO has been reported to be nonmutagenic and noncarcinogenic. In this study, we have detected the direct mutagenicity of NPRO plus natural sunlight towards Salmonella typhimurium. Furthermore, formation of 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodG), a mutagenic lesion, was observed in calf thymus DNA treated with NPRO plus simulated sunlight. The treatment with NPRO and sunlight induced single strand breaks in the superhelical replicative form of phage M13mp2 DNA. Single-strand DNA breaks also occurred in the human fibroblast cells on treatment with NPRO plus UVA, as detected by the comet assay. An analysis using scavengers suggested that both reactive oxygen species and NO radical mediate the strand breaks. The formation of nitric oxide was observed in NPRO solution irradiated with UVA. We analyzed the photodynamic spectrum of mutation induction and DNA breakage using monochromatic radiation at a series of wavelengths between 300 and 400 nm. Both mutation frequencies and DNA breakage were highest at the absorption maximum of NPRO, 340 nm. The co-mutagenic and co-toxic actions of NPRO and sunlight merit attention as possible mechanisms increasing the carcinogenic risk from UVA irradiation.
吸入香烟烟雾后内源性形成 N-亚硝基脯氨酸。
DOI：
--
发表时间：
1984
期刊：
IARC scientific publications
影响因子：
--
作者：
Brunnemann,KD;Scott,JC;Haley,NJ;Hoffmann,D
通讯作者：
Hoffmann,D
吸烟者内源性形成 N-亚硝基脯氨酸。
影响因子：
11.2
作者：
Hoffmann,D;Brunnemann,KD
通讯作者：
Brunnemann,KD
大鼠中亚硝基脯氨酸的代谢命运。
影响因子：
11.2
作者：
Chu,C;Magee,PN
通讯作者：
Magee,PN