Angiography reveals novel features of the retinal vasculature in healthy and diabetic mice
Angiography reveals novel features of the retinal vasculature in healthy and diabetic mice
复制标题
DOI：
10.1016/j.exer.2015.06.023
复制
发表时间：
2015-09-01
影响因子：
3.4
通讯作者：
Ruberte, Jesus
中科院分区：
文献类型：
--
作者：
McLenachan, Samuel;Magno, Aaron Len;Ruberte, Jesus
小鼠视网膜是研究致盲性视网膜血管疾病如糖尿病视网膜病变的发病机制和治疗的常用动物模型。在这项研究中，我们的目的是表征正常和病理变化的血管解剖在小鼠视网膜使用荧光血管造影可视化扫描激光检眼镜和光学相干断层扫描（SLO-OCT）。我们使用海德堡视网膜血管造影术（HRA）和OCT系统检查了来自C57 BL/6 J野生型小鼠以及糖尿病视网膜病变的Ins 2（秋田）和Akimba小鼠模型的眼睛。在三个焦平面上进行血管造影以检查不同的血管层。为了与血管造影数据进行比较，平行进行了体外分析，包括印度墨水血管造影、组织学和3D共聚焦扫描激光显微镜。SLO-OCT荧光血管造影可清晰显示视网膜各层血管，深血管丛（DVP）的血管密度较浅血管丛（SVP）高。建立了小动脉和小静脉类型学，并观察到小静脉类型之间的结构差异。出乎意料的是，发现玻璃体动脉在15%的C57 BL/6小鼠中持续存在，与外周视网膜毛细血管形成血管瘤。通过血管造影术在Akimba视网膜中容易检测到黄绿素渗漏，但在Ins 2（秋田）小鼠中未观察到。6月龄Ins 2（秋田）小鼠DVP的血管密度增加，而SVP显示毛细血管前小动脉的分支减少。总之，我们提出了第一个全面的表征小鼠视网膜血管SLO-OCT荧光素血管造影。使用这种临床成像技术，我们报告了以前未被认识到的变化，C57 BL/6 J血管解剖和新的特点，血管视网膜病变的Ins 2（秋田）小鼠模型的糖尿病。皇冠版权所有（C）2015由爱思唯尔有限公司出版。保留所有权利。
The mouse retina is a commonly used animal model for the study of pathogenesis and treatment of blinding retinal vascular diseases such as diabetic retinopathy. In this study, we aimed to characterize normal and pathological variations in vascular anatomy in the mouse retina using fluorescein angiography visualized with scanning laser ophthalmoscopy and optical coherence tomography (SLO-OCT). We examined eyes from C57BL/6J wild type mice as well as the Ins2(Akita) and Akimba mouse models of diabetic retinopathy using the Heidelberg Retinal Angiography (HRA) and OCT system. Angiography was performed on three focal planes to examine distinct vascular layers. For comparison with angiographic data, ex vivo analyses, including Indian ink angiography, histology and 3D confocal scanning laser microscopy were performed in parallel. All layers of the mouse retinal vasculature could be readily visualized during fluorescein angiography by SLO-OCT. Blood vessel density was increased in the deep vascular plexus (DVP) compared with the superficial vascular plexus (SVP). Arteriolar and venular typologies were established and structural differences were observed between venular types. Unexpectedly, the hyaloid artery was found to persist in 15% of C57BL/6 mice, forming anastomoses with peripheral retinal capillaries. Fluorescein leakage was easily detected in Akimba retinae by angiography, but was not observed in Ins2(Akita) mice. Blood vessel density was increased in the DVP of 6 month old Ins2(Akita) mice, while the SVP displayed reduced branching in precapillary arterioles. In summary, we present the first comprehensive characterization of the mouse retinal vasculature by SLO-OCT fluorescein angiography. Using this clinical imaging technique, we report previously unrecognized variations in C57BL/6J vascular anatomy and novel features of vascular retinopathy in the Ins2(Akita) mouse model of diabetes. Crown Copyright (C) 2015 Published by Elsevier Ltd. All rights reserved.