A microsatellite linkage map of rainbow trout (Oncorhynchus mykiss) characterized by large sex-specific differences in recombination rates.
A microsatellite linkage map of rainbow trout (Oncorhynchus mykiss) characterized by large sex-specific differences in recombination rates.
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发表时间：
2000-07
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影响因子：
3.3
通讯作者：
T. Sakamoto;T. Sakamoto;R. Danzmann;K. Gharbi;Pamela Howard;A. Ozaki;S. Khoo;Rachael A. Woram;N. Okamoto;M. Ferguson;Lars-Erik Holm;R. Guyomard;B. Høyheim
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作者：
T. Sakamoto;T. Sakamoto;R. Danzmann;K. Gharbi;Pamela Howard;A. Ozaki;S. Khoo;Rachael A. Woram;N. Okamoto;M. Ferguson;Lars-Erik Holm;R. Guyomard;B. Høyheim
我们利用三个回交家族中的 191 个微卫星、3 个 RAPD、7 个 ESMP 和 7 个同种酶标记，构建了四倍体衍生物种虹鳟鱼 (Oncorhynchus mykiss) 的遗传连锁图谱。连锁图由 29 个连锁群组成，由于男性特有的伪连锁排列，女性图中可能存在臂位移。重复微卫星标记的同线性用于识别和确认一些先前报道的基于等位酶标记的假连锁排列。使用雌核发育二倍体进行半四分体分析，鉴定了 15 个着丝粒区域（20 个染色体臂）。女性图谱长度约为 10 M，但这是一个很大的低估，因为许多基因分型片段在 LOD 阈值 3.0 时仍未分配。观察到女性：男性地图距离存在极大差异（F:M 比例，3.25:1）。女性端粒区域的重组率（0.14:1）比男性低得多，而女性在着丝粒附近区域的重组率则高得多（F:M，10:1）。假定几乎只出现在雄性中的四价结构可以解释所观察到的差异。
We constructed a genetic linkage map for a tetraploid derivative species, the rainbow trout (Oncorhynchus mykiss), using 191 microsatellite, 3 RAPD, 7 ESMP, and 7 allozyme markers in three backcross families. The linkage map consists of 29 linkage groups with potential arm displacements in the female map due to male-specific pseudolinkage arrangements. Synteny of duplicated microsatellite markers was used to identify and confirm some previously reported pseudolinkage arrangements based upon allozyme markers. Fifteen centromeric regions (20 chromosome arms) were identified with a half-tetrad analysis using gynogenetic diploids. Female map length is approximately 10 M, but this is a large underestimate as many genotyped segments remain unassigned at a LOD threshold of 3.0. Extreme differences in female:male map distances were observed (ratio F:M, 3.25:1). Females had much lower recombination rates (0.14:1) in telomeric regions than males, while recombination rates were much higher in females within regions proximal to the centromere (F:M, 10:1). Quadrivalent formations that appear almost exclusively in males are postulated to account for the observed differences.