Ultrastructural and Antigenic Properties of Neural Stem Cells and Their Progeny in Adult Rat Subventricular Zone
Ultrastructural and Antigenic Properties of Neural Stem Cells and Their Progeny in Adult Rat Subventricular Zone
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DOI：
10.1002/glia.20741
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发表时间：
2009-01-15
期刊：
影响因子：
6.2
通讯作者：
Lindvall, Olle
中科院分区：
文献类型：
--
作者：
Danilov, Alexandre I.;Gomes-Leal, Walace;Lindvall, Olle
在成年啮齿动物脑中，室管膜下区（SVZ）的神经干细胞（NSCs）持续产生嗅球中间神经元。基于它们的超微结构和抗原特性，在小鼠SVZ中已经区分了NSC、瞬时扩增前体细胞和神经母细胞（分别为B、C和A细胞）。在这里，我们的目的是确定这些细胞类型在大鼠SVZ超微结构和光学显微镜水平，并确定每种细胞类型的抗原特性，使用黄金和荧光免疫标记。我们发现具有单纤毛的星形胶质细胞（NSCs，对应于B细胞）和成神经细胞（A细胞）。我们还观察到有丝分裂细胞、室管膜细胞、移位的室管膜细胞和成熟的星形胶质细胞。相反，未检测到瞬时扩增前体细胞（C细胞）。神经干细胞和神经母细胞具有在睫状体上表达的表皮生长因子受体（EGFR）和血小板衍生生长因子受体α（PDGFR α），并且是并入增殖标记物BrdU的唯一细胞类型。在整个有丝分裂过程中，EGFR和PDGFR α与有丝分裂纺锤体的微管相关。室管膜和移位的室管膜细胞也表达EGFR和PDGFR α在其纤毛上，但不掺入BrdU。我们的研究结果表明，在成年大鼠SVZ的神经干细胞直接产生神经母细胞。在有丝分裂期间，NSC分解初级纤毛并对称地分布EGFR和PDGFR α。在他们的后代中。(C)2008 Wiley-Liss，Inc.
Neural stem cells (NSCs) in the subventricular zone (SVZ) continuously generate olfactory bulb interneurons in the adult rodent brain. Based on their ultrastructural and antigenic properties, NSCs, transient amplifying precursor cells, and neuroblasts (B, C, and A cells, respectively) have been distinguished in mouse SVZ. Here, we aimed to identify these cell types in rat SVZ ultrastructurally and at the light microscopy level, and to determine the antigenic properties of each cell type using gold and fluorescence immunolabeling. We found astrocytes with single cilia (NSCs, correspond to B cells) and neuroblasts (A cells). We also observed mitotic cells, ependymal cells, displaced ependymal cells, and mature astrocytes. In contrast, transient amplifying precursor cells (C cells) were not detected. The NSCs and neuroblasts had epidermal growth factor receptor (EGFR) and platelet-derived growth factor receptor alpha (PDGFR alpha) expressed on the ciliary apparatus and were the only cell types incorporating the proliferation marker BrdU. Throughout mitosis, EGFR and PDGFR alpha were associated with the microtubule of the mitotic spindle. Ependymal and displaced ependymal cells also expressed EGFR and PDGFR alpha on their cilia but did not incorporate BrdU. Our findings indicate that the NSCs in adult rat SVZ give rise directly to neuroblasts. During mitosis, the NSCs disassemble the primary cilium and symmetrically distribute EGFR and PDGFR alpha. among their progeny. (C) 2008 Wiley-Liss, Inc.